Kuyisibopho sethu ngempela ukwanelisa izidingo zakho futhi sikukhonze ngendlela efanele.Ukugcwaliseka kwakho kuwumvuzo wethu omkhulu kunayo yonke.Sibheke phambili ekuhambeni kwakho ukuze uthole inqubekelaphambili ehlanganyelwe ye-Factory Supply China Kit One-step Detection Virus ye-Dengue Virus (qPCR-Probe), Siphinde saba yifekthri ye-OEM eqokiwe yemikhiqizo ethengiswayo edumile emhlabeni jikelele.Siyakwamukela ukukhuluma nathi ukuze uthole ukuxoxisana nokubambisana okwengeziwe.
Kuyisibopho sethu ngempela ukwanelisa izidingo zakho futhi sikukhonze ngendlela efanele.Ukugcwaliseka kwakho kuwumvuzo wethu omkhulu kunayo yonke.Sibheke phambili ekuhambeni kwakho ukuze uthole inqubekela phambili ngokuhlanganyelaI-China Dengue Virus Isikhathi Sangempela PCR Kit, I-Dengue Virus Qpcr Kit, Njengoba siyizixazululo eziphezulu zefekthri yethu, uchungechunge lwethu lwezixazululo luhloliwe futhi lwasizuzela izitifiketi zeziphathimandla ezinolwazi.Ukuze uthole amapharamitha angeziwe nemininingwane yohlu lwezinto, qiniseka ukuthi uchofoza inkinobho ukuze uthole ulwazi olwengeziwe.

Imininingwane

50×20μl rxns, 200×20μl rxns, 1000×20μl rxns, 2000×20μl rxns

I-2×Real PCR EasyTM Mix-SYBR ehlinzekwa yi-Real Time PCR EasyTM-SYBR Green I kit iyisistimu entsha ye-premix esebenzisa i-SYBR Green I ye-Real Time PCR ukusabela kokukhulisa i-PCR, okungathuthukisa kakhulu ukucaciswa komkhiqizo nokuzwela kokusabela .I-ROX inikezwa njengodayi wokulawula wangaphakathi.Amandla e-fluorescence ale khithi aphindwe izikhathi ezingu-3-5 kunemikhiqizo efanayo, engazwela kakhulu futhi ibonise ngokunembile ukugxila kwesifanekiso se-DNA esihlosiwe.

I-2×Real PCR EasyTM Mix-SYBR iqukethe i-Taq DNA Polymerase eyingqayizivele ye-Foregene.Uma iqhathaniswa nama-enzyme e-Taq ajwayelekile, inezinzuzo zokusebenza kahle kokukhulisa i-amplification, ikhono elithile eliqinile lokukhulisa kanye nezinga lokungafani eliphansi.Inganciphisa ukukhulisa okungaqondile futhi ithuthukise ukunemba kwe-PCR.

Izingxenye zekhithi

Izici&izinzuzo

■ I-Simple—2X PCR Mix ukuze kwehliswe iphutha lokuhlola nesikhathi sokusebenza

■ Ibhafa ecacisiwe—elungiselelwe kahle kanye ne-enzyme ye-Taq eqala ukushisa ingavimbela ukukhulisa okungaqondile kanye nokwakheka kwe-primer dimer.

■ Ukuzwela okuphezulu—kukwazi ukubona amakhophi aphansi esifanekiso

■ Ukuguquguquka okuhle—kuhambisana nezinsimbi eziningi zesikhathi sangempela ze-PCR

Isicelo sekhithi

Ukuhlaziywa kwe-qPCR

Ukugeleza komsebenzi

Umfanekiso

Isitoreji kanye Nempilo yeshelufu

Le kit kufanele igcinwe kude nokukhanya futhi kufanele igcinwe ku -20 ℃.Uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ isikhathi esifushane (izinsuku eziyi-10).Kuyisibopho sethu ngempela ukwanelisa izidingo zakho futhi sikunikeze ngokufanele.Ukugcwaliseka kwakho kuwumvuzo wethu omkhulu kunayo yonke.Sibheke phambili ekuhambeni kwakho ukuze uthole inqubekelaphambili ehlanganyelwe ye-Factory Supply China Kit One-step Detection Virus ye-Dengue Virus (qPCR-Probe), Siphinde saba yifekthri ye-OEM eqokiwe yemikhiqizo ethengiswayo edumile emhlabeni jikelele.Siyakwamukela ukukhuluma nathi ukuze uthole ukuxoxisana nokubambisana okwengeziwe.
Ukunikezwa KwemboniI-China Dengue Virus Isikhathi Sangempela PCR Kit, I-Dengue Virus Qpcr Kit, Njengoba siyizixazululo eziphezulu zefekthri yethu, uchungechunge lwethu lwezixazululo luhloliwe futhi lwasizuzela izitifiketi zeziphathimandla ezinolwazi.Ukuze uthole amapharamitha angeziwe nemininingwane yohlu lwezinto, qiniseka ukuthi uchofoza inkinobho ukuze uthole ulwazi olwengeziwe.