Sisekela abathengi bethu ngemikhiqizo esezingeni eliphezulu yekhwalithi kanye nenkampani esezingeni eliphezulu.Ukuba ngumkhiqizi onguchwepheshe kulo mkhakha, sithole ulwazi olucebile lokusebenza olusebenzayo ekukhiqizeni nasekuphatheni Ifektri eyenza i-China Non-Toxic Nuclease Removal Reagents Yokukhipha Ukungcola, Siyakwamukela noma yimuphi umbuzo enkampanini yethu.Sizojabula ukusungula ubudlelwano bebhizinisi obunobungane nawe!
Sisekela abathengi bethu ngemikhiqizo esezingeni eliphezulu yekhwalithi kanye nenkampani esezingeni eliphezulu.Ukuba ngumkhiqizi onguchwepheshe kulo mkhakha, sithole ulwazi olucebile lokusebenza olusebenzayo ekukhiqizeni nasekuphatheniI-China Nucleic Acid Remover, I-Rnase ne-Dnase Scavenger, Ithini intengo enhle?Sinikeza amakhasimende ngentengo yefekthri.Esimweni sekhwalithi enhle, ukusebenza kahle kudinga ukunakwa futhi kugcinwe inzuzo efanele ephansi nenempilo.Kuyini ukulethwa okusheshayo?Senza ukulethwa ngokwezidingo zamakhasimende.Nakuba isikhathi sokulethwa sincike enanini le-oda nobunkimbinkimbi balo, sisazama ukuhlinzeka ngezinto ngesikhathi.Ngethemba ukuthi singaba nobudlelwano bebhizinisi besikhathi eside.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa i-fish caudal fin engu-1mg noma amaqanda ayi-10 ezinhlanzi.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulwa nokungcola 2× PCR EasyTM Mix (UNG), olungaqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi luqinisekise ukunemba nokunemba kokukhulisa.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Izinhlanzi ze-Zebra nezinye izinhlanzi zamanzi ahlanzekile.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

v I-Reagent Forgene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-vReagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-vReagent 2×PCR EasyTM Mix(UNG), uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe ingakapheli izinsuku eziyi-10).Sisekela abathengi bethu ngemikhiqizo yekhwalithi ephezulu nenkampani esezingeni eliphezulu.Ukuba ngumkhiqizi onguchwepheshe kulo mkhakha, sithole ulwazi olucebile lokusebenza olusebenzayo ekukhiqizeni nasekuphatheni Ifektri eyenza i-China Non-Toxic Nuclease Removal Reagents Yokukhipha Ukungcola, Siyakwamukela noma yimuphi umbuzo enkampanini yethu.Sizojabula ukusungula ubudlelwano bebhizinisi obunobungane nawe!
Ukwenziwa kwefekthriI-China Nucleic Acid Remover, I-Rnase ne-Dnase Scavenger, Ithini intengo enhle?Sinikeza amakhasimende ngentengo yefekthri.Esimweni sekhwalithi enhle, ukusebenza kahle kudinga ukunakwa futhi kugcinwe inzuzo efanele ephansi nenempilo.Kuyini ukulethwa okusheshayo?Senza ukulethwa ngokwezidingo zamakhasimende.Nakuba isikhathi sokulethwa sincike enanini le-oda nobunkimbinkimbi balo, sisazama ukuhlinzeka ngezinto ngesikhathi.Ngethemba ukuthi singaba nobudlelwano bebhizinisi besikhathi eside.