I-China Factory ngqo I-China ISO Igunyazwe i-chemistry labhorethri ama-reagents Umkhiqizi Nomphakeli |I-Foregene

Ifekthri ngokuqondile China ISO Igunyazwe ama-reagents aselabhorethri amakhemikhali

Ifekthri ngokuqondile I-China ISO Igunyazwe ama-reagents aselabhorethri yekhemistri Isithombe esifakiwe

Incazelo yekhithi:

Le kit isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic ku-zebrafish nezinye izicubu zezinhlanzi zasemanzini ahlanzekile, amaphiko omsila noma amasampula amaqanda ezinhlanzi zokusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.Inqubo yokukhipha i-DNA ye-genomic ku-lysis buffer iqedwa phakathi kwemizuzu eyi-10-30 ku-65°C.Azikho ezinye izinqubo ezidingekayo njengokususwa kwamaprotheni kanye ne-RNA, futhi i-trace DNA ekhishiwe ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.

I-2×PCR EasyTM Mix inokubekezelela okuqinile kwe-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ukuze ihlolwe njengesifanekiso sokukhulisa kahle nokuqondile.Lesi sici siqukethe i-ForegeneD-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-react buffer, i-PCR optimizer kanye nesiqinisi.Isetshenziswa ngokuhambisana ne-lysis buffer ingakwazi ukubona ngokushesha futhi kalula amasampula, futhi inezici zokuzwela okuphezulu, ukucaciswa okuqinile, nokuzinza okuhle.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Sihlala senza umsebenzi wenziwe wokuba ngabasebenzi ababambekayo ukuze siqinisekise ukuthi singakunikeza kalula ikhwalithi ephezulu kanye nenani elikhulu kakhulu leFactory ngqo I-China ISO Approvedama-reagents e-chemistry laboratory, Ngemibuzo eyengeziwe noma kufanele ube nanoma yimuphi umbuzo mayelana nemikhiqizo yethu, sicela ungangabazi ukusithinta.
Sihlala siwenza umsebenzi wokuba ngabasebenzi ababambekayo ukuze siqinisekise ukuthi singakunikeza kalula ikhwalithi ephezulu kanye nenani elikhulu kunawo wonkeama-reagents e-chemistry laboratory, Sithuthukise ubuchwepheshe bokukhiqiza, futhi siphishekela ubuhlakani obusha ezimpahleni.Ngesikhathi esifanayo, inkonzo enhle iye yathuthukisa idumela elihle.Sikholelwa ukuthi inqobo nje uma uqonda umkhiqizo wethu, kufanele uzimisele ukuba ozakwethu nathi.Ngibheke ngabomvu uphenyo lwakho.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	I-Buffer FP
	I-Foregene Protease
	I-6× I-DNA Yokulayisha Ibhafa
Ingxenye II	2×PCR Kulula^TMHlanganisa
Iziyalezo

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa i-fish caudal fin engu-1mg noma amaqanda ayi-10 ezinhlanzi.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Izinhlanzi ze-Zebra nezinye izinhlanzi zamanzi ahlanzekile.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

wk-flw-zebra-fish-direct-PCR

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

v I-Reagent Forgene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-vReagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-vReagent 2×PCR EasyTM Mix, uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).

Sihlala senza umsebenzi wenziwe ukuze sibe abasebenzi ababambekayo ukuze siqinisekise ukuthi singakunikeza kalula izinga eliphezulu kanye nenani elikhulu kakhulu leFactory ngokuqondile I-China ISO Approved 120tess/H Automatic Electrolyte Analyzer kanye neReagent Price Mslea07, Ngeminye imibuzo noma uma unombuzo mayelana nemikhiqizo yethu, sicela ungangabazi ukusithinta.
Ifekthri iqonde ngqo i-China Electrolyte Analyzer, i-Blood Gas Analyzer, Sithuthukise ubuchwepheshe bokukhiqiza, futhi siphishekela ukusungula izinto ezintsha.Ngesikhathi esifanayo, inkonzo enhle iye yathuthukisa idumela elihle.Sikholelwa ukuthi inqobo nje uma uqonda umkhiqizo wethu, kufanele uzimisele ukuba ozakwethu nathi.Ngibheke ngabomvu uphenyo lwakho.

Okwedlule: ama-reagents kanye nezinto ezisetshenziswayo zaselabhorethri

Olandelayo: lab kanye reagent materials

Bhala umyalezo wakho lapha futhi usithumelele wona