Ukuze ukwazi ukwanelisa izidingo zekhasimende ngendlela efanele, yonke imisebenzi yethu yenziwa ngokuqinile ngokuhambisana nesiqubulo sethu esithi “Ikhwalithi Ephezulu, Umaka Wamanani Ancintisanayo, Isevisi Esheshayo” ye-Factory Cheap Hot China Isitoko Esikhulu Semvelo Extract I-Myricetin 529-44-2, Ukutholakala okuqhubekayo kokuthengiswayo kwebanga elibalulekile kuhlanganiswe nokwesekwa kwethu okuqinile okuthuthukisiwe emhlabeni jikelele ngemuva kokuqinisekisa okuqinile kwemakethe yomhlaba wonke.
Ukuze sikwazi ukwanelisa izidingo zekhasimende ngendlela efanele, yonke imisebenzi yethu yenziwa ngokuvumelana nesiqubulo sethu esithi “Ikhwalithi Ephezulu, Umaka Wamanani Ancintisanayo, Isevisi Esheshayo” ye529-44-2, I-Myricetin yaseChina, Ngenhloso “yokuncintisana ngekhwalithi enhle futhi sithuthuke ngobuhlakani” kanye nomgomo wesevisi “wokuthatha ukufunwa kwamakhasimende njengokuqondiswa”, sizokwethula ngobuqotho imikhiqizo efanelekayo kanye nenkonzo enhle kumakhasimende asekhaya nawamazwe ngamazwe.

Imininingwane

50 Preps, 200 Preps

Izingxenye zekhithi

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Ukuze sikwazi ukwanelisa izimfuneko zeklayenti, yonke imisebenzi yethu yenziwa ngokuqinile ngokuvumelana nesiqubulo sethu esithi “Ikhwalithi Ephezulu, Umaki Wamanani Ancintisanayo, Isevisi Esheshayo” Yefektri Eshibhile Eshibhile I-China Enkulu I-My Stock of Natural 5 I-Continual 4 I-Continual 2 ye-Natural avava. okuthengiswayo ngokuhlanganiswa nokwesekwa kwethu okuhle kakhulu kwangaphambi nangemuva kokuthengisa kuqinisekisa ukuncintisana okuqinile emakethe ekhula ngokukhula emhlabeni jikelele.
I-Factory Cheap Hot China Myricetin, 529-44-2, Ngenhloso “yokuncintisana ngekhwalithi enhle futhi uthuthuke ngobuhlakani” kanye nomgomo wesevisi “wokuthatha ukufunwa kwamakhasimende njengokuma kwawo”, sizokwethula imikhiqizo efanelekayo kanye nenkonzo enhle kumakhasimende asekhaya nawamazwe ngamazwe ngobuqotho.