I-China iqondise ikhithi ye-PCR Umkhiqizi Nomphakeli |I-Foregene

ikhithi ye-PCR eqondile

Incazelo yekhithi:

Le kit isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic ku-zebrafish nezinye izicubu zezinhlanzi zasemanzini ahlanzekile, amaphiko omsila noma amasampula amaqanda ezinhlanzi zokusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.Inqubo yokukhipha i-DNA ye-genomic ku-lysis buffer iqedwa phakathi kwemizuzu eyi-10-30 ku-65°C.Azikho ezinye izinqubo ezidingekayo njengokususwa kwamaprotheni kanye ne-RNA, futhi i-trace DNA ekhishiwe ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.

I-2×PCR EasyTM Mix inokubekezelela okuqinile kwe-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ukuze ihlolwe njengesifanekiso sokukhulisa kahle nokuqondile.Lesi sici siqukethe i-ForegeneD-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-react buffer, i-PCR optimizer kanye nesiqinisi.Isetshenziswa ngokuhambisana ne-lysis buffer ingakwazi ukubona ngokushesha futhi kalula amasampula, futhi inezici zokuzwela okuphezulu, ukucaciswa okuqinile, nokuzinza okuhle.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Sihlala sifeza umoya wethu "wokusungula okuletha intuthuko, ukwenza izinga eliphezulu lokuziphilisa, ukukhangisa ngabaphathi kanye nenzuzo yokumaketha, amaphuzu esikweletu aheha abathengi ngekhithi ye-PCR eqondile, besibheke phambili ukubambisana nawo wonke amathemba avela ekhaya naphesheya.Ngaphezu kwalokho, ukugcwaliseka kwamakhasimende kuwumsebenzi wethu waphakade.
Sihlala sisebenzisa umoya wethu "wokusungula okuletha intuthuko, ukwenza impilo ethile yekhwalithi ephezulu, ukukhangisa kwabaphathi kanye nenzuzo yokumaketha, amaphuzu esikweletu aheha abathengiI-Direct PC Kit, Besilokhu sibheke ngabomvu ukubambisana nawe eduze ukuze izinzuzo zethu mutual kanye nentuthuko phezulu.Siqinisekise ikhwalithi, uma amakhasimende ayenganelisekile ngekhwalithi yemikhiqizo, ungabuya phakathi kwezinsuku eziyi-7 nezimo zawo zangempela.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	I-Buffer FP
	I-Foregene Protease
	I-6× I-DNA Yokulayisha Ibhafa
Ingxenye II	2×PCR Kulula^TMHlanganisa
Iziyalezo

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa i-fish caudal fin engu-1mg noma amaqanda ayi-10 ezinhlanzi.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Izinhlanzi ze-Zebra nezinye izinhlanzi zamanzi ahlanzekile.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

wk-flw-zebra-fish-direct-PCR

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

v I-Reagent Forgene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-vReagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-vReagent 2×PCR EasyTM Mix, uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).

Sihlala sisebenzisa umoya wethu "wokusungula okuletha intuthuko, ukwenza imali ethile yokuziphilisa esezingeni eliphezulu, ukukhangisa kwabaphathi kanye nenzuzo yokumaketha, Amaphuzu esikweletu aheha abathengi be-Big Discount China Cdv AG I-Canine Distemper Virus Antigen Rapid Test Kit, besilokhu sibheke ukubambisana nawo wonke amathemba avela ekhaya naphesheya.Ngaphezu kwalokho, ukugcwaliseka kwamakhasimende kuwumsebenzi wethu waphakade.
Ikhadi Lokuhlola I-Big Discount China, Imichilo Yokuhlola, Besilokhu sibheke ngabomvu ukubambisana nawe ukuze uthole izinzuzo zethu ezifanayo kanye nentuthuko ephezulu.Siqinisekise ikhwalithi, uma amakhasimende ayenganelisekile ngekhwalithi yemikhiqizo, ungabuya phakathi kwezinsuku eziyi-7 nezimo zawo zangempela.

Okwedlule: Ikhithi yokuhlukanisa ye-RNA yekhwalithi ephezulu/yobuqotho

Olandelayo: Ifekthri Iphakela ngokuqondile i-China Top Brand Passenger Tyres PCR Trailer Tire 155r12 185r14c 195r14c 205/55r16 215/45r17

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