I-China I-Wholesale yase-China I-China Yonke Isizini Ikhwalithi Engcono Kakhulu I-PCR Umkhiqizi Nomhlinzeki |I-Foregene

I-China Wholesale yase-China Yonke Isizini I-PCR Yekhwalithi Engcono Kakhulu Yezindleko

I-China Wholesale China Yonke Isizini Ikhwalithi Engcono Kakhulu ye-PCR Isithombe Esifakiwe

Incazelo yekhithi:

Le kit isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic ku-zebrafish nezinye izicubu zezinhlanzi zasemanzini ahlanzekile, amaphiko omsila noma amasampula amaqanda ezinhlanzi zokusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.Inqubo yokukhipha i-DNA ye-genomic ku-lysis buffer iqedwa phakathi kwemizuzu eyi-10-30 ku-65°C.Azikho ezinye izinqubo ezidingekayo njengokususwa kwamaprotheni kanye ne-RNA, futhi i-trace DNA ekhishiwe ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.

I-2×PCR EasyTM Mix inokubekezelela okuqinile kwe-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ukuze ihlolwe njengesifanekiso sokukhulisa kahle nokuqondile.Lesi sici siqukethe i-ForegeneD-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-react buffer, i-PCR optimizer kanye nesiqinisi.Isetshenziswa ngokuhambisana ne-lysis buffer ingakwazi ukubona ngokushesha futhi kalula amasampula, futhi inezici zokuzwela okuphezulu, ukucaciswa okuqinile, nokuzinza okuhle.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isihluthulelo sempumelelo yethu “Ikhwalithi Yomkhiqizo Enhle, Inani Elinengqondo Nesevisi Ephumelelayo” ye-China Wholesale China All Season Best Quality Cost-Effective PCR, “Quality”, “ukwethembeka” kanye “nesevisi” isimiso sethu.Ukwethembeka kwethu nokuzibophezela kwethu kuhlala kunenhlonipho enkonzweni yakho.Khuluma Nathi Namuhla Ukuze uthole imininingwane eyengeziwe, xhumana nathi manje.
Isihluthulelo sempumelelo yethu "Ikhwalithi Yomkhiqizo Enhle, Inani Elizwakalayo kanye Nesevisi Ephumelelayo" yeIkhwalithi engcono kakhulu ye-PCR, I-China All Season PCR, Ithimba lethu lizazi kahle izimfuno zemakethe emazweni ahlukene, futhi liyakwazi ukuhlinzeka ngezinto ezifanele zekhwalithi ngamanani angcono kakhulu ezimakethe ezahlukene.Inkampani yethu isivele isungule ithimba eliqeqeshiwe, elinobuhlakani nelinomthwalo wemfanelo ukuthuthukisa amakhasimende ngomgomo wokunqoba okuningi.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	I-Buffer FP
	I-Foregene Protease
	I-6× I-DNA Yokulayisha Ibhafa
Ingxenye II	2×PCR Kulula^TMHlanganisa
Iziyalezo

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa i-fish caudal fin engu-1mg noma amaqanda ayi-10 ezinhlanzi.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Izinhlanzi ze-Zebra nezinye izinhlanzi zamanzi ahlanzekile.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

wk-flw-zebra-fish-direct-PCR

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

v I-Reagent Forgene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-vReagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-vReagent 2×PCR EasyTM Mix, uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).

Isihluthulelo sempumelelo yethu “Ikhwalithi Yomkhiqizo Enhle, Inani Elizwakalayo kanye Nesevisi Ephumelelayo” ye-China Wholesale China All Season Best Quality Cost-Effective PCR 185/70r13, “Quality”, “ukwethembeka” kanye “nesevisi” kuwumgomo wethu.Ukwethembeka kwethu nokuzibophezela kwethu kuhlala kunenhlonipho enkonzweni yakho.Khuluma Nathi Namuhla Ukuze uthole imininingwane eyengeziwe, xhumana nathi manje.
I-China Wholesale China All Season PCR, i-PCR Yekhwalithi Engcono Kakhulu, Ithimba lethu lazi kahle izimfuno zemakethe emazweni ahlukene, futhi liyakwazi ukuhlinzeka ngezinto ezifanele zekhwalithi ngamanani angcono kakhulu ezimakethe ezahlukene.Inkampani yethu isivele isungule ithimba eliqeqeshiwe, elinobuhlakani nelinomthwalo wemfanelo ukuthuthukisa amakhasimende ngomgomo wokunqoba okuningi.

Okwedlule: Ifekthri Iphakela ngokuqondile i-China Rna DNA Nucleic Acid Extraction Reagent

Olandelayo: Idizayini Edumile ye-China Disposable Virus Sampling Tube ene-Swabs Yezisetshenziswa Zokuhlola

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