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Umphakeli wase-China Ikhithi Yokuhlola ye-Rt-PCR ye-Magnetic Bead Acid Yezimboni

Incazelo yekhithi:

Le kit isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic ku-zebrafish nezinye izicubu zezinhlanzi zasemanzini ahlanzekile, amaphiko omsila noma amasampula amaqanda ezinhlanzi zokusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.Inqubo yokukhipha i-DNA ye-genomic ku-lysis buffer iqedwa phakathi kwemizuzu eyi-10-30 ku-65°C.Azikho ezinye izinqubo ezidingekayo njengokususwa kwamaprotheni kanye ne-RNA, futhi i-trace DNA ekhishiwe ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.

I-2× PCR EasyTM Mix (UNG) isebenzisa i-dUTP esikhundleni se-dTTP ngesisekelo se-2× PCR EasyTM Mix, futhi yengeza i-UNG enzyme (Uracil-N-glycosylase) engalulaza ithempulethi equkethe i-dUTP ngesikhathi esifanayo.Ngaphambi kokusabela kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukuthunaza umkhiqizo we-PCR oqukethe i-uracil.I-enzyme ye-UNG ngeke ibe namuphi umthelela kusifanekiso esingaqukethe i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela amathuba okuhlolwa kofuzo okukhulu.Ukungcoliswa kwemikhiqizo ye-PCR kwenzekile.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Sineqembu elisebenza kahle kakhulu ukubhekana nemibuzo evela kumakhasimende.Umgomo wethu “ukwaneliseka kwamakhasimende okungu-100% ngekhwalithi yomkhiqizo wethu, intengo kanye nenkonzo yethu yeqembu” futhi sijabulela idumela elihle phakathi kwamakhasimende.Ngamafekthri amaningi, singahlinzeka ngezinhlobonhlobo eziningi ze-China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit, Ifemu yethu yakhula ngokushesha ngosayizi nedumela ngenxa yokuzinikela kwayo ngokuphelele ekukhiqizeni okuseqophelweni eliphezulu, amanani amakhulu ezisombululo kanye nezinsiza ezimangalisayo zamakhasimende.
Sineqembu elisebenza kahle kakhulu ukubhekana nemibuzo evela kumakhasimende.Umgomo wethu “ukwaneliseka kwamakhasimende okungu-100% ngekhwalithi yomkhiqizo wethu, intengo kanye nenkonzo yethu yeqembu” futhi sijabulela idumela elihle phakathi kwamakhasimende.Ngamafekthri amaningi, singahlinzeka ngezinhlobonhlobo eziningiIkhithi yokukhipha yaseChina, Ikhithi yokukhipha i-Nucleic Acid, Inkampani yethu ihlale igxile ekuthuthukisweni kwemakethe yamazwe ngamazwe.Manje sinamakhasimende amaningi eRussia, amazwe aseYurophu, e-USA, emazweni aseMpumalanga Ephakathi kanye namazwe ase-Afrika.Sihlala silandela ukuthi ikhwalithi iyisisekelo ngenkathi isevisi iqinisekisa ukuhlangabezana nawo wonke amakhasimende.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I

I-Buffer FP

I-Foregene Protease

I-6× I-DNA Yokulayisha Ibhafa

Ingxenye II

2×PCR KululaTMImiksi(UNG)

Iziyalezo

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa i-fish caudal fin engu-1mg noma amaqanda ayi-10 ezinhlanzi.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulwa nokungcola 2× PCR EasyTM Mix (UNG), olungaqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi luqinisekise ukunemba nokunemba kokukhulisa.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Izinhlanzi ze-Zebra nezinye izinhlanzi zamanzi ahlanzekile.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

v I-Reagent Forgene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-vReagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-vReagent 2×PCR EasyTM Mix(UNG), uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe ngaphakathi kwezinsuku eziyi-10).

Sineqembu elisebenza kahle kakhulu ukubhekana nemibuzo evela kumakhasimende.Umgomo wethu “ukwaneliseka kwamakhasimende okungu-100% ngekhwalithi yomkhiqizo wethu, intengo kanye nenkonzo yethu yeqembu” futhi sijabulela idumela elihle phakathi kwamakhasimende.Ngamafekthri amaningi, singahlinzeka ngezinhlobonhlobo eziningi ze-China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit, Ifemu yethu yakhula ngokushesha ngosayizi nedumela ngenxa yokuzinikela kwayo ngokuphelele ekukhiqizeni okuseqophelweni eliphezulu, amanani amakhulu ezisombululo kanye nezinsiza ezimangalisayo zamakhasimende.
China SupplierIkhithi yokukhipha yaseChina, Ikhithi yokukhipha i-Nucleic Acid, Inkampani yethu ihlale igxile ekuthuthukisweni kwemakethe yamazwe ngamazwe.Manje sinamakhasimende amaningi eRussia, amazwe aseYurophu, e-USA, emazweni aseMpumalanga Ephakathi kanye namazwe ase-Afrika.Sihlala silandela ukuthi ikhwalithi iyisisekelo ngenkathi isevisi iqinisekisa ukuhlangabezana nawo wonke amakhasimende.


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