5000bp DNA Ladder
Incazelo yekhithi:
◮ 2000bp DNA Ladderiwumkhiqizo olungele ukusetshenziswa futhi usuvele uqukethe i-1x Iyalayisha Ibhafa.Ungathatha ngokuqondile inani elifanele le-Marker ye-electrophoresis ngokuya ngezidingo zokuhlola.I-BM5000 DNA Marker inamabhendi e-DNA angu-8, amabhande e-DNA yilawa: 100bp(50ng/5μl), 250bp(50ng/5μl), 500bp(50ng/5μl), 750bp(75ng/5μl), 1000)ng0/5p(l), 1000)ng0/5p(l) ), 3000bp(30ng/5μl), 5000bp(50ng/5μl).
Izincazelo
Izimpawu ze-DNA ezikhiqizwe yinkampani yethu zonke zitholwa ngokugaywa kwe-enzyme yama-plasmids.Omaka abakhiqizwe yile nqubo banesizinda esihlanzekile, amabhendi acacile, ikhwalithi ezinzile futhi bangafinyelela ukulinganisa okunembile komaka.Umkhiqizo uqukethe odayi ababili (udayi we-cyan nophuzi), futhi izinga lokufuduka kwe-electrophoresis lingahlulelwa ngokushintsha kombala ngesikhathi se-electrophoresis.Izinga lokufuduka kwedayi ye-cyan lifana ne-3-5kb ku-1% yejeli ye-agarose, futhi izinga lokufuduka likadayi ophuzi limayelana Izinga lokufuduka lifana nelebhande le-50bp, futhi inqubekelaphambili ye-electrophoresis ingabonwa ngokuqondile ngeso lenyama.Kulula ukuyisebenzisa futhi isithombe se-electrophoresis sicacile.
Lo mkhiqizo uwumkhiqizo olungele ukusetshenziswa futhi usuvele uqukethe i-1x Loading Buffer.Ungathatha ngokuqondile inani elifanele le-Marker ye-electrophoresis ngokuya ngezidingo zokuhlola.I-BM5000 DNA Marker inamabhendi e-DNA angu-8, amabhande e-DNA yilawa: 100bp(50ng/5μl), 250bp(50ng/5μl), 500bp(50ng/5μl), 750bp(75ng/5μl), 1000)ng0/5p(l), 1000)ng0/5p(l) ), 3000bp(30ng/5μl), 5000bp(50ng/5μl).
Imininingwane
Inombolo Yekhathalogi Imininingwane
I-DMO-003-01 250ml×1 ithubhu
I-DMO-003-021mL×1 ithubhu
Ukugxila komkhiqizo
Ukuhlushwa kuyinto455ng/5μl
Electropherogram
Izimo zokugcina
I-4℃ ngemva kokuncibilika, -20℃ yokugcina isikhathi eside.
