Sibambelele embonweni othi "Ukudala izimpahla zekhwalithi ephezulu nokwenza ubungane obuhle nabantu namuhla abavela emhlabeni wonke", sihlala sibeka intshisekelo yabathengi ukuthi baqale ngayo ngo-2019 I-China Medical Equipment Portable Portable Real-Time PCR System System DNA/Rna Extraction Kit, sizonika abantu amandla ngokukhuluma nokulalela, nokubeka isibonelo kwabanye.
Ukunamathela embonweni "wokudala izimpahla zekhwalithi ephezulu nokwenza ubungane obuhle nabantu namuhla abavela kuwo wonke umhlaba", sihlala sibeka intshisekelo yabathengi ukuthi siqale ngayoI-China Nucleic Acid Extraction, Ikhithi yokukhipha i-Nucleic Acid, Sigcizelela "Ikhwalithi Okokuqala, Idumela Kuqala kanye Nekhasimende Kuqala".Sizibophezele ekuhlinzekeni ngemikhiqizo yekhwalithi ephezulu kanye nezinsizakalo ezinhle zangemuva kokuthengisa.Kuze kube manje, imikhiqizo yethu isithunyelwe emazweni angaphezu kuka-60 nasezindaweni emhlabeni jikelele, njengaseMelika, Australia naseYurophu.Sijabulela idumela eliphezulu ekhaya naphesheya.Sihlala siphikelela kumgomo othi "Isikweletu, Ikhasimende Nekhwalithi", silindele ukubambisana nabantu kuzo zonke izigaba zokuphila ukuze sizuze ngokufanayo.

Imininingwane

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Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Sinamathela ukuze siqonde “Ukudala izimpahla zekhwalithi ephezulu nokwenza ubungane obuhle nabantu namuhla abavela kuwo wonke umhlaba”, sihlala sibeka intshisekelo yabathengi ukuthi siqale ngayo ngonyaka ka-2019 wekhwalithi ephezulu yaseChina Medical Equipment/PCnaR abantu baya kwi-Real-Technologies ye-DNA ukuxhumana nokulalela, Ukwenza isibonelo kwabanye nokufunda kokuhlangenwe nakho.
2019 Ikhwalithi ephezuluI-China Nucleic Acid Extraction, Ikhithi yokukhipha i-Nucleic Acid, Sigcizelela "Ikhwalithi Okokuqala, Idumela Kuqala kanye Nekhasimende Kuqala".Sizibophezele ekuhlinzekeni ngemikhiqizo yekhwalithi ephezulu kanye nezinsizakalo ezinhle zangemuva kokuthengisa.Kuze kube manje, imikhiqizo yethu isithunyelwe emazweni angaphezu kuka-60 nasezindaweni emhlabeni jikelele, njengaseMelika, Australia naseYurophu.Sijabulela idumela eliphezulu ekhaya naphesheya.Sihlala siphikelela kumgomo othi "Isikweletu, Ikhasimende Nekhwalithi", silindele ukubambisana nabantu kuzo zonke izigaba zokuphila ukuze sizuze ngokufanayo.