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Izixazululo Ezine Eziyinhloko Zokulawula Ukungcola Komkhiqizo we-PCR
1:Miselela izinto zokuhlola ngesikhathi Setha (NTC) isilawuli esinegethivu bese ukuphinda izikhathi eziningi.Uma sekutholakele ukuthi kukhona ukungcoliswa komkhiqizo we-PCR elabhorethri, buyisela zonke izinto zokuhlola ngesikhathi.Okufana nokuthi: hlanza kabusha futhi ulungise iziqalo, phinda ugcwalise ithiphu le-pipette, E...Funda kabanzi -
Ama-enzyme amabili e-RT-PCR asebenza kabili
Imibhalo yendabuko ehlanekezelwe ayikwazi ukubekezelela izinga lokushisa eliphezulu (izinga lokushisa elilungile lomsebenzi we-MMLV ngu-37-50°C, futhi i-AMV ingu-42-60°C).I-RNA yegciwane eyinkimbinkimbi ayikwazi ukuhlehliswa ngempumelelo ibe yi-cDNA emazingeni okushisa aphansi, okuholela ekusebenzeni kahle kokutholwa Ukuncipha.Tra...Funda kabanzi -
Ubuchwepheshe be-nucleic acid isothermal amplification
I-PCR iwubuchwepheshe bokukhulisa i-nucleic acid obusetshenziswa kakhulu futhi busetshenziswa kakhulu ngenxa yokuzwela nokucaciswa kwayo.Kodwa-ke, i-PCR idinga ukukhishwa kwe-thermal okuphindaphindiwe futhi ayikwazi ukususa imikhawulo yokuthembela kumathuluzi nemishini, ekhawulela ukusetshenziswa kwayo emitholampilo ...Funda kabanzi -
Uyini umgomo we-mRNA?
Uyini umgomo we-mRNA Umuthi wokugoma we-mRNA udlulisela i-RNA kumaseli omzimba ukuze uveze futhi ukhiqize ama-antigen amaprotheni ngemva kokuguqulwa okufanelekile kwe-in vitro, ngaleyo ndlela kuholele umzimba ukuthi ukhiqize impendulo yokuzivikela komzimba ngokumelene ne-antigen, ngaleyo ndlela andise i-bod...Funda kabanzi -
Ubuchwepheshe be-PCR
I-PCR (i-polymerase chain reaction) ingenye ye-in-vitro DNA amplification technologies, enomlando ongaphezu kweminyaka engama-30.Ubuchwepheshe be-PCR basungulwa ngu-Kary Mullis wase-Cetus, e-USA ngo-1983. U-Mullis wafaka isicelo se-PCR patent ngo-1985 futhi washicilela iphepha lokuqala lezemfundo le-PCR leSayensi ngokufanayo...Funda kabanzi -
Ukutholwa kwe-coronavirus entsha, kusuka ku-nucleic acid, i-antibody kuya ku-antigen, ungakukhetha kanjani ukujwayelekile kwalolu bhubhane?
Ubhubhane seluguqule umhlaba.Emhlabeni wonke, ohulumeni bawo wonke amazwe babhekene nezinselele ezinkulu ekuvimbeleni nasekulawuleni ubhubhane.Ngesikhathi sobhadane lwe-COVID-19, i-China isezigabeni ezine zohlaka lokuvimbela kanye nokusabela (ukuvimbela, ukutholwa, ukulawula kanye nokhiye wokuxazulula inkinga ...Funda kabanzi -
Izindlela ezine zokuvimbela ukungcoliswa kwemikhiqizo ye-PCR
Ukwakha I-SOP Systerm Sungula isilingo se-PCR SOP ukuze ulinganise ukuziphatha kwabasebenzi bokuhlola.Abahloli bathobela izinqubo zokusebenza ngokuqinile, futhi banciphise ukungcoliswa kwe-PCR okungase kubangelwe izici zomuntu noma kuvimbele ukwenzeka kokungcola emsebenzini.Ngaphezu kwalokho...Funda kabanzi -
Ungakuthuthukisa kanjani ukusebenza kahle kokubhalwe phansi kwe-LncRNA?
I-Lead Long non-coding RNA, i-lncRNA iyi-RNA engafaki amakhodi enobude obungaphezu kwama-nucleotide angu-200, ngokuvamile phakathi kuka-200-100000 nt.I-lncRNA ilawula ukuvezwa kofuzo emazingeni e-epigenetic, okulotshiweyo kanye nangemuva kokuloba, futhi ibamba iqhaza ekuthuliseni i-X chromosome, ukuphrinta kwe-genome kanye ne-chroma...Funda kabanzi -
Ingadluliswa isuka kumuntu iye komunye ngemizuzwana eyi-14.Kwenzekeni kuhlobo “oluthathelana kakhulu” lweDelta eZhong Nanshan Pass?
Umthombo ohumushiwe: Umhleli wethimba le-WuXi AppTec E-Guangzhou, e-China, amaphoyisa anesibopho sokusiza uphenyo lwesifo esiwumshayabhuqe akhiphe ividiyo egadayo: Endaweni yokudlela efanayo, laba ababili bangena endlini yokugezela belandelana ngaphandle kokuthintana ngokomzimba.Imizuzwana engu-14 kuphela ye-co-e...Funda kabanzi -
Ukulebula kwamangqamuzana e-SNP nokutholwa
Ngemva kokuba isazi saseMelika u-Eric S. Lander ehlongoza ngokusemthethweni i-nucleotide polymorphism (SNP) njengemaki yamangqamuzana esizukulwane sesithathu ngo-1996, i-SNP iye yasetshenziswa kabanzi ekuhlaziyeni isimilo sesimo sezomnotho, ukwakhiwa kwemephu yokuxhumanisa izakhi zofuzo, kanye nokuhlolwa kwezakhi zofuzo zabantu., D...Funda kabanzi -
Isayensi Edumile |Ungayithola kanjani i-coronavirus entsha nge-qPCR
I-COVID-19 isifo esithathelwanayo esibangelwa i-Severe Acute Respiratory Syndrome Coronavirus Type 2. Uma umuntu ethelelekile, izimpawu ezivame kakhulu zihlanganisa imfiva, ukukhwehlela, nokuphefumula.Amasampula asetshenziselwa ukuhlolwa angaqoqwa ngama-nasopharyngeal swabs noma ama-oropharyngeal swabs.Yini mina...Funda kabanzi -
Ingabe usasebenzisa ubuchwepheshe obasungulwa eminyakeni engu-30 edlule ukukhipha i-RNA?
Ingabe usasebenzisa i-Trizol ukuze ukhiphe i-RNA?Gosh, ungangivumeli ngikhathazeke ngempilo yakho!U-Trizol usezokhishwa inyumbazane, ungangitsheli ukuthi usasebumnyameni?Ingxenye eyinhloko ye-Trizol reagent yi-phenol, futhi ama-organic reagents afana ne-chloroform nawo ayangezwa ku-experimen...Funda kabanzi
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