Ukwakha Uhlelo lwe-SOP

Sungula isilingo se-PCR SOP ukuze ulinganise ukuziphatha kwabasebenzi bokuhlola.

Abahloli bathobela izinqubo zokusebenza ngokuqinile, futhi banciphise ukungcoliswa kwe-PCR okungase kubangelwe izici zomuntu noma kuvimbele ukwenzeka kokungcola emsebenzini.Ngaphezu kwalokho, ohlolayo kufanele abe nolwazi oluhambisanayo lobungcweti namakhono, okuhlanganisa ubungoti bemishini ehlobene nokusebenza, ukucacisa yonke inqubo yomsebenzi, ukuphatha izindlela zokwelapha zokungcoliswa nezindlela zokulawula ikhwalithi zaselabhorethri, kanye nokukwazi ukuchaza kahle imiphumela yokuhlolwa.

Ukwakha Ilabhorethri Ye-PCR Ejwayelekile

Ilabhorethri ye-PCR ihlukaniswe yaba izindawo ezine ngokomgomo, okuyindawo yokulungisa i-reagent, indawo yokucubungula isampula, indawo yokukhulisa, kanye nendawo yokuhlaziya umkhiqizo.Izindawo ezimbili zokuqala yizindawo zokwandiswa kwangaphambili, kanti izindawo ezimbili zokugcina yizindawo zokwandiswa.Indawo yokukhulisa i-pre-amplification kanye ne-post-amplification zone kufanele ihlukaniswe ngokuqinile.Izinto zokuhlola, ama-reagents, iphepha lokuqopha, amapeni, izinto zokuhlanza, njll. zingageleza kuphela ukusuka endaweni yokukhulisa ngaphambili ukuya endaweni ye-post-amplification, okungukuthi, ukusuka endaweni yokulungiselela i-reagent, indawo yokucubungula isampula, indawo yokukhulisa, kanye nendawo yokuhlaziywa komkhiqizo wokukhulisa, futhi akumele igeleze ibheke emuva.Ukugeleza komoya elabhorethri kufanele futhi kugeleze ukusuka endaweni yokukhulisa isandiso ukuya endaweni yangemuva kokukhulisa, hhayi ukuya emuva.

Yehlisa izinyathelo zokuhlola

Uma ilabhorethri ihlola kuphela i-PCR nokuhlonza, kuyanconywa ukuthi kusetshenziswe i-fluorescent quantitative PCR esikhundleni se-PCR evamile.

Imiphumela ye-PCR yobuningi be-Fluorescence ingaqoqwa futhi ihlaziywe ngamasignali e-fluorescent, ngakho-ke asikho isidingo sokuvula isivalo se-electrophoresis ngemva kokusabela, esigwema ukungcoliswa kwemikhiqizo ye-PCR okubangelwa ukuvuza kwemikhiqizo yokusabela ukuze yakhe ama-aerosol.Uma ukhuphula inani lokuvuleka kwe-cap ngesikhathi sesinyathelo sokulayisha i-gel electrophoresis, ukungcoliswa kwe-aerosol kungenzeka.Kunconywa ukukhuthaza ukusetshenziswa kwe-PCR yomthamo futhi kancane kancane kuthathelwe indawo i-PCR yekhwalithi.

Adopt UNG anti-pollution system

Isistimu yokungcoliswa komkhiqizo we-UNG elwa ne-PCR isetshenziselwa ukusabela kwe-PCR.

Uhlelo lusebenzisa i-dUTP esikhundleni se-dTTP.Ngemva kokusabela kwe-PCR, yonke imikhiqizo ye-PCR (izingcezu ze-DNA) ihlanganiswa ne-dUTP;emzuliswaneni olandelayo wokusabela kwe-PCR, i-enzyme ye-UNG engezwe kusistimu ifakwa ku-37°C imizuzu engu-5 ngaphambi kwe-PCR, okungase kucace ukuthi Nciphisa zonke izingcezwana ze-DNA eziqukethe i-dUTP, bese yenza ukusabela kwe-PCR.Lokhu kungasusa ngokuphelele ukungcoliswa kwe-aerosol okubangelwa imikhiqizo ye-PCR.Umphumela uboniswa emfanekisweni ongezansi:

Qaphela: Ochungechungeni lwe-PCR oluqondile, ungakhetha uchungechunge lwemikhiqizo yohlelo lokungcoliswa komkhiqizo olulwa ne-PCR lwe-FJ Biotech.

Phakamisa

Ezilabhorathri ezihlola i-genotyping enkulu, kunconyelwa kakhulu ukusebenzisa uhlelo lokungcolisa umkhiqizo we-UNG anti-PCR ukuhlola izinto ezisebenza ngokusebenza kabusha ngokungeziwe ekwakhiweni kwamalabhorethri anengqondo.

Isikhumbuzi: Ukusetshenziswa kwalolu hlelo akukwazi ukususa ukungcoliswa komkhiqizo we-PCR osekudaliwe.Ngakho-ke, uhlelo lwe-UNG kufanele lusetshenziswe ekuqaleni kokuhlolwa okufanele, futhi uhlelo lwe-UNG kufanele ngaso sonke isikhathi lusetshenziselwe ukukhulisa i-PCR, ukuze kuvinjelwe ukungcoliswa kwemikhiqizo ye-PCR.Okungelona iqiniso.

Kunconywa ukusebenzisa i-Direct PCR-UNG uhlelo lwe-Forge Biotech lapho uhlola izinga elikhulu, njengokuthi:Plant Leaf Direct PCR Kit-UNG;

Plant Seed Direct PCR Kit-UNG;

I-Animal Tissue Direct PCR Kit-UNG;

I-Mouse Tail Direct PCR Kit-UNG;

I-Zebra Fish Direct PCR Kit-UNG.

Lolu chungechunge lwamakhithi avela ku-Foregene alukwazi nje ukwenza ukutholwa kwe-PCR ngokushesha nangesilinganiso esikhulu, kodwa futhi luvimbela ngempumelelo futhi lulawule ukungcoliswa komkhiqizo we-PCR.