Ingemuva
Eminyakeni yamuva, ama-extracellular vesicles (EVs) adonse ukunaka kwabantu njengethuluzi lokwelapha elingase libe khona;Nokho, umphumela wokwelapha we-EVs ku-endometriosis awukabikwa.I-Endometriosis yisifo esivamile esingesona esiyingozi esithinta u-10-15% wabesifazane abaneminyaka yobudala yokuzala, okubangela izimpawu ezihlukahlukene, okuholela ekwehleni kwezinga lempilo kanye nomthwalo omkhulu womphakathi.
Isingeniso Sesihloko
Ngomhla zingama-20 kuNtulikazi, 2021, iqembu locwaningo likaSolwazi Wang Guoyun waseSibhedlela sase-Qilu saseShandong University lashicilela iphepha lokucwaninga elinesihloko esithi “M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting Development of Endometriosis” on Frontiers in immunology, elalixoxa ngokuncipha kwe-M2.Ukuba nokwenzeka kwe-nanovesicles (NVs) ekwelapheni i-endometriosis.
Lesi sihloko sisebenzisa indlela ye-extrusion eqhubekayo ukulungiselela ama-M1NV, futhi sisebenzisa indlela ye-co-culture ukutadisha izinguquko ku-angiogenesis, ukufuduka, ukuhlasela nezinye izinkomba zamaseli e-eutopic endometrial stromal (EM-ESCs) ezigulini ezine-endometriosis.Ngesikhathi esifanayo, imodeli yegundane ye-endometriosis yasungulwa, futhi amagundane aphathwa nge-PBS, i-MONVs noma i-M1NVs, ngokulandelanayo, ukuze kuhlolwe ukusebenza nokuphepha kwe-M1NV ekwelapheni i-endometriosis.
Imiphumela yabonisa ukuthi i-in vitro M1NVs ingavimbela ngokuqondile noma ngokungaqondile ukufuduka nokuhlasela kwama-EM-ESC, futhi ivimbele i-angiogenesis.Kumodeli yegundane, ama-M1NV avimbela ukwenzeka kwe-endometriosis ngohlelo lwe-M2 macrophage kabusha ngaphandle kokudala ukulimala kwesitho.Kubonisa ukuthi ama-M1NV anganqanda ngokuqondile ukwenzeka kwe-endometriosis, futhi angavinjelwa ngokuphindaphinda ama-macrophage ohlobo lwe-M2 ohlotsheni lwe-M1.Ngakho-ke, ukusetshenziswa kwe-M1NV kungase kube indlela entsha yokwelapha i-endometriosis.
Foregene Usizo
Ocwaningweni, ngenxa yokuthi i-M1NV yalungiswa ngokucindezela ngokuqhubekayo ama-macrophage e-M1, isihloko sasebenzisa i-qRT-PCR ukuze kutholwe izici ezibangela ukuvuvukala kanye nezimpawu ze-macrophage ze-M1 iNOS, TNF-a kanye ne-IL-6 mRNA ku-M1NV kanye ne-M1 macrophages.Ukuphindaphinda okuhlobene koshintsho.Imiphumela yabonisa ukuthi ama-M1NV aqukethe izimpawu eziningi ze-pro-inflammatory factor mRNA kanye ne-M1 macrophage, ebonisa ukuthi ama-M1NV angakwazi ukugcina ngokuphumelelayo izici zokusebenza zamaseli e-M1.Le ndlela yocwaningo isebenzisa i-QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I ye-Foregene.
I-Cell Direct RT-qPCR Kitimininingwane

Izimo zohlelo lokusebenza:
 
1. Ukulawulwa kofuzo kanye nokuhlaziywa kwenkulumo, ukuqinisekiswa kwe-gene overexpression noma umphumela wokuphazamiseka, ukuhlolwa kwezidakamizwa, njll.;
2. Ukutholwa kwesimo sofuzo samangqamuzana okunzima ukukhuleka njengamangqamuzana ayinhloko, ama-stem cells, namangqamuzana ezinzwa;
3. Ukutholwa kwe-mRNA kumasampuli afana nama-exosomes nama-nanovesicles.
Izici: