Isimo samanje se-monkeypox virus
Ngokwezindaba ze-WHO ngoJuni 7, emasontweni amathathu nje kuphela (Meyi 13 kuya kuJuni 7), sekubikwe amacala angaphezu kuka-1,000 27 emazweni nasezifundeni ezingezona izinkawu.I-WHO ikholelwa ukuthi amacala angase athathwe kancane ngenxa yolwazi olulinganiselwe lwe-epidemiological and laboratory, futhi amacala angase avele nakwamanye amazwe.Kepha le nhlangano ithe isagcina ukuhlolwa “okulinganiselwe” kwengozi yezempilo yomphakathi emhlabeni jikelele ngokuqubuka kwemonkeypox.
I-Monkeypox, isifo se-zoonotic esibangelwa i-monkeypox virus (MPXV), ibhekwa njengesifo esibaluleke kakhulu esiphethwe yi-orthopoxvirus kubantu kusukela "kuqedwa" kwe-smallpox.Igciwane lemonkeypox liyakwazi ukudluliselwa komuntu kusuka kumuntu kuya kumuntu ngezinto eziphilayo ezigulayo, uketshezi lomzimba, amaconsi okuphefumula, nezinto ezingcolisayo, kodwa lo mzila wokudlulisela ngokuvamile udinga ukuthintana okuseduze isikhathi eside.
I-Monkeypox virus igciwane le-DNA elinemicu ephindwe kabili elingakhula esikweni kumaseli ezinso zezinkawu eziluhlaza zase-Afrika, okubangela imiphumela ye-cytopathic.Igciwane lingatholwa ngokuhlolwa okuningana: i-enzyme-linked immunosorbent assay (ELISA), ukuhlolwa kokutholwa kwe-antigen, ukuhlolwa kwe-polymerase chain reaction (PCR), noma ukuhlukaniswa kwegciwane ngesiko lamaseli.
Isithombe esivela kuwebhusayithi
Silapha ukuze sikusize ngokugqashuka kwemonkeypox
Njengomphakeli wasekhaya wocwaningo lwebhayoloji yamangqamuzana kanye nezixazululo zokuxilonga ze-in vitro, i-Foregene Biotech izibophezele ekusizeni abacwaningi bezokwelapha nabesayensi ukuthi bahlonze futhi bacwaninge igciwane lemonkeypox ngokushesha nakalula.I-Foregene Biotech inobuchwepheshe obuhamba phambili bokubopha i-DNA kuphela/i-RNA kuphela kanye nobuchwepheshe be-Direct PCR, ikunikeza inhlobonhlobo yocwaningo lwesayensi nezinto ezingasetshenzisiwe ze-IVD zocwaningo lwakho nokuxilonga igciwane lemonkeypox.
Izincomo zomkhiqizo wocwaningo lwesayensi#
✿ Ukuhlanzwa okusebenzayo kanye nokukhipha i-viral DNA ne-RNA
✿ Ukuhlanzeka okuphezulu, akukho ukungcola
✿ Ayikho i-DNase ne-RNase esetshenziswayo, futhi umkhiqizo awehliswanga
✿ Hlangana nezinhlelo zokusebenza ezihlukene zokuhlola ezansi nomfula
✿ Ingasetshenziselwa ukuhlaziywa kobuningi bezifanekiso nge-fluorescence quantitative PCR, futhi ingasetshenziselwa ukutholwa kwe-allele.
✿ Uhlelo lwe-PCR oluhlukile olunokuhambisana okuqinile
✿ Isebenzisa i-Foregene HS Taq Polymerase ene-hot-start-start, inekhono eliphezulu lokukhulisa i-amplification, ukuzwela nokucacisa.
#IVD isincomo somkhiqizo#
✿ Isetshenziselwa ukwelashwa kusengaphambili kwesampula okufanele ihlolwe, ukuze i-nucleic acid ezohlolwa kusampula ikhishwe esimweni sokubopha nezinye izinto, futhi ingasetshenziswa nemikhiqizo ye-Foreasy Direct PCR/RT-qPCR ukuze kutholakale ukutholakala ngokushesha.
✿ Ukusebenza okulula, isivinini sokucubungula okusheshayo, ukuphuma okuphezulu, asikho isidingo sesistimu yokukhipha, ukonga isikhala nokuthuthukisa ukusebenza kahle
✿ Ifanele ukucaciswa okuphezulu kwe-PCR, ukutholwa kwe-PCR ye-multiplex
✿ Ifanele ukukhuliswa kwesifanekiso se-genome esinokuqukethwe okuphezulu kwe-GC (>60%), isakhiwo sesibili esiyinkimbinkimbi kanye nokutholwa kokukhulisa i-genome ngezinga elikhulu
✿ Inomsebenzi wokuqalisa oshisayo, ukukhulisa ngokushesha nokuthembeka okuqinile
Isikhathi sokuthumela: Jun-10-2022
