Kunezinhlobo ezimbili eziyinhloko zobungozi kulabhorethri ye-PCR: ubungozi be-biosafety kanye nobungozi bokungcoliswa kwe-nucleic acid.Okwangaphambili kulimaza abantu kanye nemvelo, futhi lokhu kwakamuva kuthinta imiphumela yokuhlolwa kwe-PCR.Lesi sihloko simayelana nezindawo zokuqapha ubungozi zelabhorethri ye-PCR kanye namazinga ahambisanayo engcuphe alethwe kuwe.

01 Ukuhlukaniswa kwelabhorethri ye-PCR

1. Ilabhorethri yokuhlola i-molecular biology

Ngokuvumelana nezidingo ze-athikili 1.1 Yezimo Eziyisisekelo Zokusetha Amalabhorethri Okuhlola Izakhi Zofuzo Zomtholampilo, amalabhorethri e-PCR ngokuvamile ahlanganisa izindawo ezine: indawo yokugcina izinto ezisetshenziswayo kanye nendawo yokulungiselela, indawo yokulungiselela isifanekiso, indawo yokukhulisa, kanye nendawo yokuhlaziya umkhiqizo.Uma kusetshenziswa indlela ye-PCR ye-fluorescent yesikhathi sangempela, indawo yokukhulisa kanye nendawo yokuhlaziya ingahlanganiswa ibe yindawo eyodwa;uma i-PCR analyzer ezenzakalelayo isetshenziswa ngokugcwele, indawo yokulungiselela isampula, indawo yokukhulisa kanye nendawo yokuhlaziya ingahlanganiswa ibe yindawo eyodwa.

I-“Workbook for New Coronavirus Nucleic Acid Testing in Medical Institutions (Trial Version 2)” ibeka ukuthi ngokomthetho, amalabhorethri ahlola i-coronavirus nucleic acid entsha kufanele amise lezi zindawo ezilandelayo: indawo yokugcina izinto ezisebenza kuyo kanye nendawo yokulungiselela, indawo yokulungiselela isifanekiso, indawo yokukhulisa nokuhlaziya umkhiqizo.Lezi zindawo ezintathu kufanele zizimele ngokuphelele komunye nomunye endaweni ebonakalayo, futhi angeke kube khona ukuxhumana okuqondile nomoya.

2. Igumbi lokulungiselela isampula

Nakuba amasampula angalungiswa kalula endaweni yokulungiselela isifanekiso, igumbi lokulungiselela isampula elikhethekile lisadingeka lapho kubhekwana namasampula ayinkimbinkimbi kanye nenani elikhulu lamasampuli.Igumbi lokulungiselela isampula linengozi enkulu yokuphepha kwezinto eziphilayo kanye nokungcoliswa kwe-nucleic acid.

3. Igumbi lokuhlanza imfucuza

Ukwelashwa kwemfucuza okungafanele kuzoletha nezingozi ezinkulu zokungcoliswa kwe-biosafety kanye ne-nucleic acid elabhorethri.Ngakho-ke, igumbi lokuhlanza imfucuza lidinga ukugadwa njalo.

02 Izindawo zokuqapha ubungozi kumalabhorethri e-PCR

Amalabhorethri ahlukene ahlukaniswe abe yigumbi lokulungiselela isampula, indawo yokugcina i-reagent kanye nendawo yokulungiselela, indawo yokulungiselela isifanekiso, indawo yokukhulisa nokuhlaziya umkhiqizo, kanye negumbi lokuhlanza imfucuza.

Ngokusho kohlobo lwesayithi lokusampula, ihlukaniswe phezulu, insimbi, isampula, ukuqapha kwemvelo kanye ne-pipette.

Izinga lobungozi lisukela kokuphansi kuye phezulu ukusuka enkanyezini eyodwa ukuya kwezintathu ★★★.

1. Igumbi lokulungiselela isampula:

Isetshenziselwa ukubhaliswa, ukulungiswa kanye nokwenza amasampula angasebenzi, futhi ubungozi bokuphepha bebhayoloji buphezulu kakhulu.Ngenxa yokuthi amasampula awakhishwa futhi akhuliswe, ngaphandle kwama-pipettes avame ukuhlangana namasampuli, ingozi yokungcoliswa kwe-nucleic acid kwezinye izingxenye iphansi.

1-4 Ukuthatha amasampula ezindaweni zokuqapha

5-8 Isampula endaweni yokuqapha

9-12 iphuzu lokuqapha amasampula

1. Indawo yokugcina neyokulungiselela i-reagent:

Isetshenziselwa ukulungiswa kwama-reagents okugcina, ukukhishwa kwama-reagents kanye nokulungiswa kwengxube yokusabela kwe-amplification, kanye nokugcinwa nokulungiswa kwezinto ezisetshenziswayo njengamashubhu e-centrifuge namathiphu we-pipette.Akukho ukuthintana okuqondile namasampuli futhi akukho nucleic acid enhle kule ndawo, ngakho-ke ingozi ye-biosafety kanye nengozi yokungcoliswa kwe-nucleic acid iphansi.

13-16 Amasampula ezindaweni zokuqapha

17-22 Isampula ekuqashweni
3. Amaphuzu wokulungiselela isampula

Isetshenziselwa ukuvula umphongolo wokudlulisa, uvale isifanekiso (uma sisebenza), ukukhipha i-nucleic acid bese uyengeza kushubhu yokusabela kwe-amplification, njll. Le ndawo ingase ihlanganise ukucutshungulwa nokuvulwa kwamasampuli, ubungozi bokuphepha kwebhayoloji buphezulu, futhi ukukhishwa kwe-nucleic acid kuyenziwa, futhi ingozi yokungcoliswa kwe-nucleic acid iphakathi nendawo ukuya phezulu.

29 Ukuthatha amasampula ezindaweni zokuqapha

4. Indawo yokukhulisa nokuhlaziya umkhiqizo:

Isetshenziselwa ukukhulisa i-nucleic acid.Le ndawo ayibandakanyi ukucutshungulwa kwesampula, futhi ubungozi bokuphepha bebhayoloji buphansi.I-nucleic acid amplification ikakhulukazi kule ndawo, futhi ingozi yokungcoliswa kwe-nucleic acid iphezulu kakhulu.

38 Ukuthatha amasampula ezindaweni zokuqapha
5. Igumbi lokuhlanza imfucuza:

Isetshenziselwa ukucubungula ingcindezi ephezulu yamasampuli.Izingozi zokuphepha kwebhayoloji ezihilelekile ekucutshungulweni kwamasampula kule ndawo ziphezulu uma kuqhathaniswa.Imikhiqizo yokukhulisa i-nucleic acid iyanconywa ukuthi iphathwe njengodoti wezokwelapha.Ukucindezela okuphezulu akunconywa, futhi ingozi yokungcoliswa kwe-nucleic acid iphansi.

43-44 Amasampula ezindaweni zokuqapha

03 Sebenzisa

Kulokhu sibale izindawo zokuqapha ezingama-44.Kulinganiselwa ukuthi abantu abaningi kufanele babuze, ingabe kudingeka benze amaphuzu amaningi kangaka?Yebo, kwenze konke!Ngiphakamisa ukuthi uqale wenze ukuhlolwa kobungozi kwelabhorethri yakho, okungenziwa ngokuya ngobungozi obusuka phezulu kuye phansi, ungakwazi futhi ukuqapha uhlobo olufanayo lwamasampula ndawonye, ​​noma ungakha uhlelo lwamasampula lokuqapha njalo.Ngamafuphi, ilabhorethri ngayinye ingenza uhlelo lwayo lokusebenzisa ngokusekelwe esimweni sayo.Ingozi enkulu yokuhlola amalabhorethri ukungazinaki ubungozi.