Amakhasimende eSikole Sesayensi Yempilo, Inyuvesi yaseSichuan ishicilele amaphepha anamaphuzu aphezulu kusetshenziswa imikhiqizo ye-Foregene, enomthelela we-17.848

Muva nje, ithimba le-Song Xu eliphuma eSikoleni SezeSayensi Yezokuphila sase-Sichuan University lishicilele uqwembe olunesihloko esithiI-coagulation factor VII, IX kanye no-X angamaprotheni e-antibacterial ngokumelene ne-Gram-negative Bacteria emelana nomuthi Ocwaningweni Lweseli.

I-Cell Research yijenali yamazwe ngamazwe eshicilelwe ngokuhlanganyela yi-Chinese Academy of Sciences kanye ne-British Nature Publishing Group, enegunya emhlabeni wezemfundo.

Lapho lesi sihloko sesishicilelwe, ngokushesha sabangela ukuzwela kwezemfundo.Kuze kube manje, imiphumela yocwaningo yamukelwe inqwaba yemithombo yezindaba efana neXinhua News Agency, World Wide Web, Phoenix Net, Southern Metropolis Daily,I-Biological Valley, i-British Daily Mail, i-American Daily Science, i-EurekAlert1!, i-Springer Nature, i-Phys.org, njll., i-BioMedCentral kanye namanye amajenali aziwayo anemibiko ebanzi, futhi ukunaka komhlaba wonke kulo mphumela wocwaningo kusakhula.

I-athikili yabonisa ukuthi izici ezintathu ze-coagulation VII, IX kanye no-X ezidlala indima ekuqalisweni kwe-coagulation cascade ziwuhlobo olusha lwe-endogenous host antibacterial protein, okungukuthi, izici ze-coagulation VII, IX kanye no-X zinezindima ezibalulekile enqubweni yokuhlangana.Ingase futhi ikwazi ukulwa namabhaktheriya e-Gram-negative, okuhlanganisa “amagciwane amakhulu” amelana kakhulu njenge-Pseudomonas aeruginosa ne-Acinetobacter baumannii.

USong Xu, umbhali ohambisana nalesi sihloko, uthe: “Esikhathini esedlule, bekukholelwa ukuthi izici zokuhlangana zingadala i-thrombosis, kodwa lolu cwaningo lwembula ukuthi izici zokuhlangana nazo zinomthelela okhethekile wokuvalwa inzalo.Lesi yisikhathi sokuqala sokutholwa ekhaya naphesheya.”

Isizinda Socwaningo

Njengoba sonke sazi, ukumelana namagciwane sekuyinkinga enkulu yezempilo yomphakathi emhlabeni wonke.Idatha efanelekile ikhomba ukuthi cishe abantu abayizigidi eziyi-1 babulawa izifo ezibangelwa amagciwane angazweli emishanguzweni emhlabeni wonke unyaka nonyaka.Uma singekho isixazululo esingcono, isibalo sabantu abafa unyaka ngamunye kusukela ngo-2050 sizoba yizigidi eziyishumi.

Ukusetshenziswa kabi kwemithi elwa namagciwane, kuhambisana nekhono elihle kakhulu lokuziphendukela kwemvelo kwebhaktheriya, kwenze amanye amagciwane e-pathogenic abengabulawa imithi elwa namagciwane angakwazi ukumelana nemithi, acishe abe “ama-super bacteria” angashabalali.

Ngaphezu kwalokho, uma kuqhathaniswa ne-Gram-positive bacteria (Gram+), amagciwane angemahle (Gram-) anzima kakhulu ukuwabulala ngenxa yokuba khona kolwelwesi lwangaphandle (ingxenye eyinhloko yi-LPS, i-alias endotoxin, i-lipopolysaccharide).I-membrane yangaphandle iyimvilophu eyakhiwe ulwelwesi lwamangqamuzana angaphakathi, udonga lweseli elincanyana kanye nolwelwesi lwamaseli olungaphandle.

Umlando wocwaningo

Ithimba likaSong Xu belicwaninga ngomthelela wezinto ezijiyayo ekwelapheni izimila eziyingozi, kodwa ngo-2009, kwatholakala kungalindelekile ukuthi izici zokuhlangana zingabulala amagciwane.Ukuze kuchazwe indlela ye-bactericidal ye-coagulation factor, iphrojekthi isineminyaka eyi-10 kusukela ekuqaleni kocwaningo kuya ekushicilelweni kwephepha.

Kutholwe ngengozi

Ngo-2009, abacwaningi bathola ngephutha ukuthi i-coagulation factor VII ingalwa ne-Escherichia coli phakathi kwezici ezingaphezu kweshumi nambili zokuhlangana.

I-Escherichia coli ingeyamagciwane e-Gram-negative kuma-bacteria.Lolu hlobo lwamabhaktheriya kunzima ukubhekana nalo, ngoba amangqamuzana awo anolwelwesi lwamangqamuzana angaphakathi, udonga lwamaseli amancane kanye nolwelwesi lwamangqamuzana lwangaphandle.Imvilophu ingagcina imishanguzo ingangeni futhi ivikele amagciwane “ekungeneni.”

Phakamisa ukucabangela

I-coagulation factor iyiqembu lamaprotheni egazini abandakanyeka ekuhlulekeni kwegazi.Lapho ukulimala komzimba womuntu kubangela ukopha, izici ezihlukahlukene zokujiya zisebenza isinyathelo ngesinyathelo ukuze zakhe imicu ye-fibrin, evala isilonda kanye nama-platelet.Uma i-coagulation factor eyodwa noma eminingana ishoda, ukuphazamiseka kwe-coagulation kuzokwenzeka.

Ososayensi baye baqaphela ukuthi iziguli ezine-coagulopathy zivame ukuthambekela ezifweni ezibangelwa amagciwane njenge-sepsis ne-pneumonia.Lokhu kuxhumana kwabaholela ekucabangeni ukuthi izici ze-coagulation zingase zingagcini nje indima ebalulekile enqubweni ye-coagulation, kodwa zingase zibe nomphumela wokulwa nokutheleleka.

Ucwaningo olunzulu

Ukuze kuphenywe ukuthi izici ze-coagulation zingabhekana nohlu olubanzi lwamagciwane e-Gram-negative, abacwaningi baqala ukutadisha indlela yayo yokulwa namagciwane ngokujulile.Bathole ukuthi i-coagulation factor VII kanye nezici ezifanayo ngokwesakhiwo i-IX kanye ne-factor X, lawa maprotheni amathathu angadabula imvilophu eqinile yamagciwane angenayo igram.

Izinto eziningi ezikhona ezilwa namagciwane ziqondise kumetabolism yeseli noma ulwelwesi lwamaseli, kodwa lezi zici ezintathu zokuhlangana zinezindlela ezihlukene zokwenza.Bangakwazi ukwenza i-hydrolyze i-LPS, ingxenye eyinhloko yolwelwesi lwangaphandle lwebhaktheriya.Ukulahlekelwa yi-LPS kwenza kube nzima ukuthi amagciwane e-Gram-negative aphile.

Qhubeka

Ithimba labacwaningi liqhubekile lahlola indlela futhi lathola ukuthiiphrotheni ye-coagulation factor isebenza kubhaktheriya ngokusebenzisa ingxenye yayo yeketanga elikhanyayo, kuyilapho ingxenye yeketanga elisindayo ingenawo umphumela wokulwa namagciwane.

Endaweni yesiko lelabhorethri, abacwaningi babona ngokucacile ukuthi ngemva kokwengeza isici se-coagulation noma izingxenye zayo zamaketanga okukhanya, imvilophu yeseli yebhaktheriya yonakaliswa kuqala, bese kuthi kungakapheli amahora angu-4, lonke iseli lebhaktheriya lacishe labhujiswa ngokuphelele.

Engeza ingxenye ye-Factor VII yochungechunge lokukhanya ku-Escherichia coli ekhulisiwe,

Izingxenye ze-membrane zangaphandle ze-bacterium zonakalisiwe, amaseli ayabhujiswa

Hhayi kuphela i-Escherichia coli, kodwa amanye amagciwane ahlolwe i-Gram-negative nawo “anqotshiwe”, okuhlanganisa i-Pseudomonas aeruginosa kanye ne-Acinetobacter baumannii.Womabili la magciwane abalwe yi-World Health Organization (WHO) njengamagciwane ayi-12 ayingozi kakhulu empilweni yabantu ngenxa yokungazweli emishanguzweni yawo.

Ukuqinisekisa kokuhlola

Ukuhlola okulandelayo kwezilwane kuqhubeke kwaqinisekisa ukusebenza kahle kwezinto ezijiyisa amabhaktheriya amakhulu.

Abacwaningi bajove amagundane ngenani elikhulu le-Pseudomonas aeruginosa engazweli emthini noma i-Acinetobacter baumannii.Ngemva kokujova imithamo ephezulu ye-factor VII light chain, amagundane asinda;kuyilapho amagundane eqenjini elilawulayo ajovwe ngosawoti ovamile ayengama-24 Bonke babulawa ukutheleleka ngemva kwamahora.

Ngemuva kokutheleleka nge-super bacteria, ukumnika kwe-factor VII light chain

Ingadlala indima yokuvikela futhi ithuthukise kakhulu izinga lokusinda lamagundane

Ukubaluleka

Njengamanje, ayikho into elwa namagciwane eyaziwa ukuthi iyasebenza nge-hydrolyzing LPS.

Ukucacisa indlela ye-antibacterial esekelwe ku-LPS hydrolysis kanye nezici zokulwa namagciwane ezici zokuhlangana, kuhlanganiswe nekhono lokukhiqiza lezi zici zokuhlangana ngesilinganiso esikhulu ngenani eliphansi, kungase kunikeze isu elisha elingabizi kakhulu lokulwa nama-bacterium ane-Gram-negative angangeni emuthini Inkinga yezempilo yomphakathi ephuthumayo iqalile.

Ngaphezu kwalokho, lo msebenzi nawo unamathemba okusebenza abanzi ekusebenzeni komtholampilo.Njengamanje, azikho izidakamizwa ezaziwayo ezilwa namagciwane ezidlala umphumela nge-hydrolyzing LPS.Ukuhlanganisa izakhiwo ze-antibacterial ze-FVII, FIX, ne-FX ngokumelene ne-LPS kanye nokukhiqizwa kwenani elikhulu elingabizi kakhulu, kulindeleke ukuthi kuthuthukiswe imithi emisha ngokumelene nezifo "ze-super bacteria".

Isandiso sesihloko

Nakuba abantu bejwayelene kakhulu negama elithi “super bacteria”, igama labo elinembile kufanele kube “amabhaktheriya amelana nezidakamizwa eziningi”, okubhekisela ohlotsheni lwamagciwane amelana nama-antibiotic amaningi.

Njengoba kushiwo ngaphambili, ukwanda kwamanje kwamagciwane kubangelwa ikakhulukazi ukusetshenziswa okungenangqondo noma ngisho nokuhlukumeza ama-antibiotics.Isibonelo, ukusetshenziswa kwe-high-frequency ye-antibiotic ebanzi ekwelapheni izifo zepheshana lokuphefumula.

Ukutheleleka ngomgudu wokuphefumula yisifo sonke esisijwayele.Ngokwezibalo, ingane ngayinye itheleleka izikhathi ezingaba ngu-6 kuya kweziyisi-9 ngonyaka, futhi intsha kanye nabantu abadala bangenwa igciwane izikhathi ezi-2 kuya kwezine ngonyaka.

Ngenxa yokuthi izifo zokuphefumula zokuphefumula ngokuvamile ziyiminyango ephuthumayo, ubunzima obukhulu kodokotela abaphuthumayo lapho bebhekene neziguli ukuthi abakwazi ukuthola ulwazi lwe-pathogenic ngesikhathi esifushane.Ngakho-ke, ukuncipha kokuhlolwa kwe-pathogenic kwenza odokotela basebenzise ama-antibiotic abanzi (okungasebenza ngempumelelo).Ngezinhlobo eziningi zamabhaktheriya).

Yile ndlela yokwelapha “yokusakaza inetha elikhulu” eye yaholela enkingeni eyandayo yokungazweli emishanguzweni etholwe amagciwane.Ngoba lapho iningi lezinhlobo ezizwelayo zibulawa ngokuqhubekayo, izinhlobo ezimelana nomuthi zizophindaphindeka ukuze zithathe indawo yezinhlobo ezizwelayo, futhi izinga lokumelana kwebhaktheriya emuthini lizoqhubeka nokukhula.

Ngakho-ke, uma umbiko onembile wokuthola i-pathogen ungatholakala ngesikhathi esifushane ukuze uqondise odokotela ekunikezeni imithi efanele, ukusetshenziswa kwama-antibiotic abanzi kungancishiswa kakhulu, ngaleyo ndlela kunciphise inkinga yokumelana namagciwane.

Libhekene nale nkinga engokoqobo, ithimba labacwaningi besayensi le-Fuji laqala ukwakha ikhithi yokuhlonza igciwane lokuphefumula lezinto eziyi-15.

Le khithi yamukela inhlanganisela ye-Direct PCR kanye nobuchwepheshe be-multiplex PCR, obukwazi ukubona i-Streptococcus pneumoniae, i-Staphylococcus aureus engamelana ne-methicillin, i-Haemophilus influenzae namanye amapheshana angu-15 avamile okuphefumula aphansi esikhwehlela cishe ngehora elingu-1.Amabhaktheriya e-Pathogenic angakwazi ukuhlukanisa ngokuphumelelayo phakathi kwamabhaktheriya ekoloni (amabhaktheriya avamile) namabhaktheriya e-pathogenic.Ngikholwa ukuthi kuzolindeleka ukuthi kube yithuluzi elisebenzayo ukusiza odokotela ekusetshenzisweni kwemithi okunembayo.

Lapho sibhekene “ne-super bacteria”, isitha somphakathi sabantu bonke, isintu asikaze sikuthathe kalula.Emkhakheni wesayensi yezempilo, kusenabacwaningi abaningi abafana nethimba lika-Song Xu abasebenza kanzima ukuze bahlole futhi basebenze buthule emgwaqeni ukuze bathole izixazululo “zamagciwane amakhulu”.

Lapha, egameni lontanga bebhayoloji kanye nabahlomuli, i-Fortune Biotech ithanda ukuzwakalisa inhlonipho yayo enkulu kubo bonke ososayensi abaye banikela ngemizamo yabo kanye nezithukuthuku zabo kulokhu, futhi bathandazela ukuthi abantu banqobe “amagciwane amakhulu” ngokushesha ngangokunokwenzeka futhi babe nempilo ephephile futhi enempilo.ezizungezile.