Isingeniso:

Ama-molecule e-RNA angasebenzisana kakhulu namanye ama-molecule ebhayoloji, anjengamaprotheni, i-DNA ne-RNA, futhi kanjalo akhona kumaseli nezinto eziphilayo ngendlela yama-macromolecular complexes.Phakathi kwazo, i-RNA-protein complexes kukhona kanye nokusebenza kwe-RNA Enye yezinhlobo ezibalulekile ze.Ukudonsela phansi kwe-RNA kuyindlela yakudala yokuhlola nokuhlonza ama-molecule e-RNA, ikakhulukazi amaprotheni asebenzisanayo ama-molecule e-lncRNA, futhi aqashelwa iningi labacwaningi namajenali ezemfundo agunyaziwe.Namuhla i-FOREGENE izokwabelana nawe ngesimiso sokuhlola kanye nenqubo yobuchwepheshe yokudonsela phansi kwe-RNA, mhlawumbe ungayisebenzisa.

1. Umgomo wokuhlola we-RNA wokudiliza ubuchwepheshe:

Ukulotshwa kwe-in vitro kanye nokuhlanganiswa kwezingcezu zethagethi ezinelebula ye-biotin ye-lncRNA noma ye-lncRNA (kuhlanganise nezingcezu ze-RNA ezisetshenziswa njengezilawuli ezinhle nezingezinhle), futhi zifukamele ngokukhipha amaseli;sebenzisa ubuhlalu obuzibuthe be-avidin-anchored ukuqoqa i-RNA-protein noma i-RNA-RNA complexes, Ngemva kokugeza ngokuphelele, inkimbinkimbi iyasuswa.Uma ithola i-molecule ye-protein target esebenzisana ne-lncRNA, yenza i-protein electrophoresis emkhiqizweni we-RNA-donsela phansi, thola iphrotheni eqondiwe ngamabala ayisiliva, futhi usike ijeli nge-mass spectrometry;futhi enze ngokuqondile ukuqinisekiswa kwe-western blot kumkhiqizo wokudonsela phansi;uma kutholwa Kumamolekyuli aqondiwe e-RNA asebenzisana ne-lncRNA, imikhiqizo ye-RNA-donsela phansi ingaphansi kokukhipha i-RNA futhi ukutholwa kwe-qRT-PCR ethile kuyenziwa.

2. I-RNA yehlisa inqubo yokuhlola ubuchwepheshe:

3. Isevisi yezobuchwepheshe ye-RNA idonsela phansi i-FOGENE:

Isiko lamaseli

Ukulungiswa kwengqikithi yeseli/i-nuclear/plasma protein extract

I-RNA yokwehlisa ukuhlolwa

I-SDS-PAGE electrophoresis, ibala lesiliva

I-mass spectrometry kanye nokuhlolwa kwe-WB

Umbiko wokuhlola

4. Icala lokuhlola

Isibalo esilandelayo sibonisa imiphumela yokudonsela phansi kwe-RNA ye-FJ Bio-Bio kanye nokutholwa kwe-electrophoresis ye-SDS-PAGE, lapho umcibisholo obomvu umaka ibhendi yomehluko:

Iqembu locwaningo likaSolwazi Song Xu waseNyuvesi yaseSichuan liqongelele iminyaka engu-15 yokuqongelela isikhathi eside emkhakheni wocwaningo lwe-lncRNA.I-Foregene Bio kanye neqembu locwaningo likaSolwazi Song Xu babambisane ukwethula ubuchwepheshe bokudonsela phansi kwe-RNA be-lncRNA, obuzokunikeza umphumela ogculisayo wocwaningo lwakho.