Kungakhathaliseki ukuthi ungumfundi osanda kuzalwa, noma ungumphathi omkhulu onamakhono aqinile namandla, uke wazihlupha ngokukhipha isamba seseli yekhwalithi ephezulu ye-RNA?Bangaki abantu abamosha amasampula ayigugu ngenxa yokusebenza kanzima futhi kanzima kwendlela yendabuko ye-Trizol?

Manje, ivangeli liyeza!Valelisa ngokuphelele ezindleleni zendabuko ezidla isikhathi nezingasebenzi kahle, futhi ukhiphe kalula i-RNA yekhwalithi ephezulu emizuzwini eyi-16.

Ikhithi Yokuzihlukanisa Yeseli Ye-RNA

Uhlelo alunayo i-RNase, asikho isidingo sokukhishwa kwe-chloroform, akukho amaprotheni nokunye ukungcola, ngakho ungaqiniseka.

Kungakhathaliseki ukuthi ungumuntu osanda kuzalwa omncane noma ungumphathi omkhulu, uzoba nezindinganiso zakho eziphakeme zekhwalithi yokukhishwa kwe-RNA.

Ngakho hlobo luni lwekhithi ikhithi ekahle?

Ngamafuphi, kusho “okuningi nokungcono.”

Akunankinga, ukwanelisa ukuhaha kwakho okuncane.

Le khithi ingakhipha i-RNA ephelele kumaseli akhuliswe kumapuleti angu-96, 24, 12, kanye nangu-6 anemithombo ephezulu.Kunezinhlobo eziningi kakhulu ze-RNA etholiwe.Ngaphezu kwezingcezu ezivamile ze-28s kanye ne-18s, ama-5s nezingcezu ezincane ze-miRNA nazo zingasetshenziswa.Kuqoqiwe.

Ikholomu eyingqayizivele yokuhlanza i-DNA ikuvumela ukuthi ususe i-DNA ye-genomic ngaphandle kokwengeza i-DNase, futhi umphumela uba ngcono, uhlangabezana nezidingo zakho zokuhlola ezikhangayo.

Ngokubona lapha, ingabe wonke umuntu ususiwe?Ungakhathazeki, umkhiqizo omuhle, khuluma namaqiniso!!!

一, Kulula kakhulu!(Imizuzu eyi-16 ayikhulumi nje)

二、Ikhwalithi ephezulu!(Umphumela uyabonakala)

I-Foregene Cell Total RNAIsolation Kits Imiphumela yokuqhathanisa yendlela yekholomu ye-adsorption kanye nendlela ye-Trizol

三、Ukuzinza okwengeziwe!Uma usuyisebenzisile izothi inhle)

Iphethini ye-Foregene ye-QPCR yofuzo A ngemva kokuloba okuhlanekezelwe

Amathiphu:

Uma inani lamaseli liphansi kakhulu, inani eliphelele le-RNA lizoncishiswa, futhi i-spectrophotometer ayikwazi ukunquma ngokunembile inani le-OD.Kunconywa ukwenza ukuhlolwa kwekhwalithi ngokuhlolwa okulandelayo kwe-qPCR.Lo mkhiqizo usebenza kangcono nge-RT EasyTM II yenkampani yethu (i-cDNA synthesis yokuqala ye-Real Time PCR) (RT-01022)!

Khetha i-Foregene Cultured Cell Total RNA Extraction Kit ukuze wenze ukuhlola kwakho isinyathelo esisodwa kusheshe.Ulindeleni, thatha isinyathelo manje!