Ngaphezu kwengxenye ka-2022, woza uzobona ukuthi yiziphi izindatshana ezinamaphuzu aphezulu ezithunyelwe ngu-Foeregene!
01
IF:17.694
Ishicilelwe ngu: The First Affiliated Hospital,Sun Yat-sen University
Okuqukethwe okuyinhloko: I-Esophageal squamous cell carcinoma (ESCC) iwuhlobo oluncane lomdlavuza womminzo.Ukuhlonza okuhlosiwe kwezokwelapha okusebenzayo kubaluleke kakhulu ekwelapheni okungcono kwe-ESCC.Olunye ucwaningo lubonise ukuthi ukulungiswa kabi kwe-RNA kungase kukhuthaze ukuvezwa nokuhunyushwa kwama-mRNA ahlobene nomdlavuza, kodwa indlela yamangqamuzana ihlala ingacacile.Lolu cwaningo lubonise ukuthi amaprotheni ayinkimbinkimbi ahlobene ne-tRNA m7G methyltransferase i-METTL1 kanye ne-WDR4 akhuliswe kakhulu ezicutshini ze-ESCC futhi aba nomthelela ekuqaguleni okubi kwe-ESCC.Kusukela lapho, izifundo ze-METTL1 kanye ne-WDR4 ezihlobene ne-WDR4 kumagundane ayimodeli ziye zaqinisekisa ukuthi umsebenzi othuthukisiwe we-tRNA m7G methyltransferase uholela ekulawulweni okuphezulu kokuvezwa kwalawa maprotheni amabili, okubuye kukhuthaze ukwanda kwamaseli e-ESCC.Lolu cwaningo lubonisa umsebenzi obalulekile we-oncogenic wokuguqulwa okungalungile kwe-tRNA m7G ku-ESCC futhi luphakamisa ukuthi ukukhomba i-METTL1 kanye nezindlela zayo zokusayina ezingezansi kungase kuthembise izinhloso zokwelapha zokwelashwa kwe-ESCC.
Umkhiqizo osizwayo:
Igundane Umsila Direct PCR Kit Foregene#TP-01333
Isixhumanisi sangempela:
https://www.nature.com/articles/s41467-022-29125-7
02
Uma: 14.903
Ishicilelwe: ILabhorethri Eyinhloko Yesifunda Yocwaningo Lwekhwalithi Emithi YaseShayina, Isikhungo Sesayensi Yezokwelapha YaseShayina, Inyuvesi yaseMacau
Okuqukethwe okuyinhloko:
Ngokuthuthukiswa kobuchwepheshe bokwenziwa, i-mRNA isiphenduke isigaba esisha semithi yokwelapha.Kodwa-ke, ukuzuza ukulethwa kwezidakamizwa ze-mRNA okusebenzayo kuseyinselele ngenxa yokuzwela kwe-mRNA kuma-RNases.Lolu cwaningo luphakamisa uhlelo olulula lokulethwa kwe-polyphenol-conjugated nanostructures engaletha ngempumelelo i-IL-10 mRNA in vivo.Ngemuva kokusebenzisa le nanostructure ukuletha izidakamizwa ze-mRNA, amazinga e-IL-10 ayelawulwa ngokuqondile, avimbela ngokuphumelelayo ukubonakaliswa kwezici ezivuthayo, ukukhuthaza ukulungiswa kwe-mucosal, ukuvikela amangqamuzana e-colonic epithelial kusuka ku-apoptosis, nokuvimbela i-dextran sodium sulfate-induced acute and chronically disease imodeli ye-murine colitis yokuvuvukala imodeli entsha yokuvuvukala imodeli ye-murine colitis ephawulekayo inemiphumela emisha yokuvuvukala.
Umkhiqizo osizwayo:
I-Animal Total RNA Isolation Kit Foregene#RE-03011
Isixhumanisi sangempela:
03
IF:12.499
Ishicilelwe ngu: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University
Okuqukethwe okuyinhloko:
Ukucindezelwa kwe-oxidative kwesidoda kuyisici esikhulu esiholela ekungazali kwabesilisa, ngakho-ke kuyindlela enokwenzeka nephumelelayo yokusungula amamodeli ezilwane zokungazali wesilisa ngokulungisa uhlelo lwe-antioxidant ngendlela eqondiwe.Kodwa-ke, izindlela ze-antioxidant eziqondene negciwane wesilisa azikacaciswanga.Lolu cwaningo luqinisekisile ukuthi isakhi sofuzo se-LanCL1 siwufuzo oluthile lwe-antioxidant emangqamuzaneni amagciwane wesilisa, futhi ukubonakaliswa kwaso kuyancishiswa noma kuhlotshaniswe nokungazali komuntu.Amagundane esilisa antula isakhi sofuzo se-LanCL1 abonisa ukulimala kwe-sperm oxidative kanye nokukhubazeka kokuzala.Enqubweni yokuhlukaniswa kwesidoda samangqamuzana egciwane, uma ukuthambekela kwe-oxidation kwenzeka, iphrotheni 1 yesici esikhethekile (SP1) izolawula ukuvezwa kofuzo lwe-LanCL1, ngaleyo ndlela ivimbele inqubo ye-oxidation yesidoda.Lolu cwaningo lubonisa ukuthi indlela ye-SP1-LanCL1 idlala indima ebalulekile ekulawuleni i-homeostasis yamasende kanye nokuzala kwabesilisa okuqondiswa ibhalansi ye-redox, futhi iphakamisa ukuthi amagundane ashintshwe i-LanCL1 anezinhlelo zokusebenza ezikhangayo njengemodeli yesilwane yokungazali kwabesilisa.
Umkhiqizo Osizwayo:
I-Mouse Tail DNA Mini Kit Foregene#DE-05211
Isixhumanisi sangempela:
http://europepmc.org/article/MED/35469022
◮Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.
◮Isidingo sesampula sincane, thatha nje imbewu eyodwa.
◮ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.
◮Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.
Ikhithi Yokuhlukanisa Yezilwane Ephelele ye-RNA
◮Asikho isidingo sokukhathazeka ngokucekelwa phansi kwe-RNA.Lonke uhlelo alunayo i-RNase-Free.
◮Susa i-DNA ngempumelelo usebenzisa Ikholomu Yokuhlanza I-DNA.
◮Susa i-DNA ngaphandle kokwengeza i-DNase.
◮Lula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi.
◮Ngokushesha—ukusebenza kungaqedwa ngemizuzu engu-30.
◮Iphephile—asikho isisetshenziswa esiphilayo esisetshenzisiwe.
◮Ukuhlanzeka okuphezulu—OD260/280≈1.8-2.1.
◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.
◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.
◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.
◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.
◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.
◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.
Isikhathi sokuthumela: Sep-13-2022
