Ukukhishwa kwe-Nucleic acid kuyikhono lesethulo lokuhlolwa kwebhayoloji yamangqamuzana.Ukuthi ingakwazi ukusebenzisa kahle leli khono kahle futhi sekube yimfuneko yokuba abahloli bakamuva bathuthukele ekubeni ongcweti bokuhlola.
Ngokwezinto ezahlukahlukene zokucwaninga, abahloli bahlukaniswa kakhulu babe yizinhlobo ezintathu ezilandelayo:
Isikhulu sezilwane, Isikhulu sezitshalo, iMicrobiology enkulu
Uhlobo ngalunye lokuhlola luzoqhubeka nokukopisha amakhophi ukuze kuhlakulele isethi yamakhono akhethekile kumasampuli athile.
Namuhla, umhleli ufuna ukuletha izisetshenziswa nezisetshenziswa ezingatholakali emgodini kwabahloli.Ngazo, ungathuthukisa ngokushesha kuzo zonke izinkomba.Akulona iphupho ukuba unkulunkulu wokuhlola.
Le nkinga ingeyabahloli bezitshalo abangochwepheshe (abahloli beminye imisebenzi nabo bangafunda kuyo~).
Ngikholwa ukuthi zonke izizukulwane zabahloli bazoke bezwe ngezindlela zakudala zokukhipha i-nucleic acid yezitshalo ezidluliswa isizukulwane kuye kwesinye isizukulwane kubanduleli bazo.Amagama amathathu amakhulu embonini yokukhipha izitshalo.
Nakuba izinsizwa ezinkulu zizinhle kakhulu, zisenamaphutha angenakugwenywa.
Indlela ye-TRIzol
Ngokuvamile kusetshenziselwa ukukhipha
I-RNA
●Kunobuthi futhi kuyaxaka
●Iqukethe ukungcoliswa kofuzo
Indlela ye-CTAB
Ngokuvamile kusetshenziselwa ukukhipha
i-genomeI-DNA
●Kudla isikhathi
● Okunzima kakhulu ukususa i-CTAB ngokuwasha kunosawoti, inani elincane lezinsalela lizoba nomthelela omkhulu emsebenzini wama-enzyme.
Indlela ye-SDS
Ngokuvamile kusetshenziselwa ukukhipha
i-plasmid
●Ayizilungele izitshalo ezinokuqukethwe okuphezulu kwe-polysaccharide
●Ukungcola kwamaprotheni
Kulo mphakathi wanamuhla onokuphindaphinda okuphindwe kabili, izindlela zendabuko zokusebenza azisakwazi ukuhlangabezana nezidingo zabahloli, futhi ukuthuthukiswa kwemishini sekuseduze.
I-Foregene Nucleic Acid Extraction Kit ihlolwe izikhathi ezingu-N ngabahloli abaningi.Kulula ukuyisebenzisa, ayisebenzisi noma yimaphi ama-reagents anobuthi, futhi ayifaki noma yimaphi ama-enzyme.I-nucleic acid etholakalayo ingokuhlanzeka okuphezulu, okubalulekile ekusebenzeni kwansuku zonke kokukhipha abahloli abahle kakhulu.
Ikhithi ye-Foregene RNA Isolation
Lapha, ngizophinde ngidalule indlela yokusebenza efihliwe kubahloli, okuwukukhetha indlela efanele yokukhipha ngokusho kohlobo lwesitshalo (indlela yokunquma uhlobo lwesitshalo isekupheleni kwesihloko!), Ngoba amanye ama-polysaccharides ezitshalo nama-polyphenols aphakeme ngokuqukethwe, futhi izindlela zendabuko ziyabulala.Akwanele ukulawula.
Hhayi-ke, kunjena...
Indlela ye-TRIzol iguqe ngokuqondile ngaphansi kwamasampula anamazinga aphezulu e-polysaccharides nama-polyphenols njengamaqabunga kabhanana, amaqabunga e-ginkgo namaqabunga kakotini.
Ngale njongo, i-Foregene ithuthukise amathuluzi amabili asebenzayo abahloli.Ikhithi yokukhipha i-RNA yesitshalo enokuqukethwe okuphansi kwe-polysaccharide kanye ne-polyphenol nokuqukethwe kwe-polysaccharide ephezulu kanye ne-polyphenol kungabulala izitha ngendlela eqondiwe, ukuze abahloli bangabe besaba izitshalo ezahlukene.Vele ushintshe imishini ngqo!
Ngaphezu kwesidingo sokuthuthukisa indlela yokukhipha, imisebenzi yesampula yokucubungula ngaphambilini nayo idinga ukuthuthukiswa.
Ngenxa yesakhiwo esikhethekile samasampula ezitshalo, izimpande neziqu eziqinile zezinye izitshalo kuba nzima kakhulu ukugaya nokuchoboza ngokulinganayo.
Embonini yokugaya, cishe amaphesenti angu-80 abantu basebenzisa i-nitrogen ewuketshezi ukuze bagaye, kuyilapho abangaphansi kuka-20% basebenzisa ukugaya ngogesi nokugaya ubuhlalu.
Nakuba ukugaya i-nitrogen ewuketshezi kushibhile futhi kulula ukukusebenzisa, kuyiphupho elibi kubahloli abanesidingo esikhulu sesampula ukugaya isikhathi ngasinye nomzamo!Uma udaka lungahlanzwa, luzodala ukungcoliseka phakathi kwamasampuli.
Umthelela wokugaya ugesi uyahlukahluka kumuntu nomuntu, futhi imikhuba yokusebenza yabahloli izoba nomthelela omkhulu kubuqotho be-nucleic acid, futhi ukusebenza kwezinga lokushisa eliphansi kuyadingeka uma kunesidingo.
ngakho, ukugaya ubuhlalu oscillation kungase kubhekwe njengendlela yamanje elula futhi ephumelelayo imishini ukugaya.
Izindlela ezivamile zokuzulazula zihlanganisa ukuzungeza okuqondile okuqondile, i-oscillation evundlile ephindaphindayo kanye ne-three-dimensional (i-oscillation evundlile).Kodwa-ke, i-crusher yethishu ye-Foregene igqekeza imodeli ekhona futhi isebenzisa indlela entsha ye-4D yokuzungeza ukwenza ukugaya kube okufanayo.
Hlangana nezidingo zesampula ezahlukahlukene, kulula ukuzisebenzisa
Ubuchwepheshe bokuchoboza be-4D obuhlukile, ukusebenza kahle kokuchoboza kukhuphuka ngo-20%
Isampula livalwe ngokuphelele, akukho ukungcoliswa okuphambene
Kuthule, ≤56 decibel
Qhathanisa imiphumela yokuhlolwa negagasi ~
Isampula yokuhlola: 50mg amaqabunga kakolo (okuqukethwe okuphansi kwama-polysaccharides nama-polyphenols)
Khipha uhlobo lwe-nucleic acid: RNA
Inani lendawo: 5μl
Ikhithi yokukhipha esetshenzisiwe yi-Foregene Plant Total RNA Extraction Kit (RE-05011)
Uma kuqhathaniswa idatha kanye negrafu ye-electrophoresis, kungabonakala ukuthi ukugxiliswa nokuhlanzeka kwe-RNA ekhishwe ngemva komshini wokugaya kuphezulu kunaleyo yokugaya i-nitrogen ewuketshezi.Ngesikhathi esifanayo, ngoba umshini wokugaya ungaphula isampula ngokuphelele, i-RNA encane ingakhishwa kangcono futhi ubuqotho be-RNA bungagcinwa.
Isampula yokuhlola: 50mg amaqabunga e-ginkgo (i-polysaccharide ephezulu nokuqukethwe kwe-polyphenol)
Khipha uhlobo lwe-nucleic acid: RNA
Inani lendawo: 5μl
Ukusebenzisa i-Foregene Polysaccharide Polyphenol Plant Total RNA Extraction Kit (RE-05021)
Bheka leli qembu lamasampula anokuqukethwe okuphezulu kwama-polysaccharides nama-polyphenols.Umphumela uba sobala nakakhulu.Ngokuhlanzeka okufanayo, ukugcwala kwe-RNA yokugaya umshini cishe izikhathi ezi-5 kune-nitrogen ewuketshezi yokugaya, futhi i-RNA encane isabonakala kancane ~
Uma unomuzwa wokuthi akukho mishini engenhla eshesha ngokwanele, khona-ke umhleli kufanele aqhamuke nemishini yokugcina!
Plant Direct PCR series
Isici esiyingqayizivele sale mishini ukuthi amaqabunga esitshalo noma imbewu ayidingi ukugaywa futhi iphuke, futhi iphonswe ngqo ku-EP tube ye-lysis, futhi ingxube ye-lysis ewumphumela ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.Kulula njengesinyathelo esisodwa ngokushesha!Ngesikhathi esifanayo, uchungechunge lwe-PCR oluqondile lwe-Foregene Plants lufakwe uhlelo lwe-UNG lokulwa nokunukubezeka, ukuze abahloli bangabe besakhathazeka ngokungcoliswa kwe-aerosol elabhorethri futhi bangathatha isilingo kalula.
Amathiphu wokwahlulela:
Indlela yokwahlulela ukuthi ngabe isitshalo se-polysaccharide polyphenol
Thatha cishe i-20mg ye-thishu block bese uyifake ku-1.5ml centrifuge tube ehlanzekile, engeza isixazululo se-200ul 200mM NaOH, uyibeke ku-95 ℃ nge-10min, bheka ukushintsha kombala ngemva kwe-lysis.
1. Uma umbala we-lysate uluhlaza noma uphuzi ngokukhanyayo, kusho ukuthi okuqukethwe kwezicubu ze-polyphenols kuphansi, futhi kunconywa ukusebenzisa uchungechunge lwe-Foregene Plant.
2. Uma i-lysate insundu ngokuphuzi noma ngokubomvu ngokunsundu, kusho ukuthi okuqukethwe kwezicubu ze-polyphenol kuphezulu, futhi kunconywa ukusebenzisa i-Foregene Polysaccharide Polyphenol Plant Plus Series.
Isikhathi sokuthumela: Aug-20-2021
