Kuze kube nguJuni 25, 2021, iKhomishini Yezempilo Kazwelonke YaseChina ikhiphe imininingwane ekhombisa ukuthi bangaphezu kwezigidi ezingama-630 abantu abagonyiwe ezweni lami, okusho ukuthi izinga lokugoma labantu bonke eChina leqe ama-40%, okuyisinyathelo esibalulekile ekusunguleni ukuzivikela komhlambi.

Ngakho-ke abantu abaningi bazokhathazeka ngokuthi bazi kanjani uma sebekhe amasosha omzimba ngemva kokuthola umuthi omusha wokugomela umqhele?

Njengamanje, ikhithi etholakala kakhulu yokutholwa kwe-antibody yomqhele emakethe ikhithi yokuthola i-antibody ye-IgM/IgG (indlela yegolide ye-colloidal).

ICoronavirus (COV) umndeni omkhulu wamagciwane adala izifo ezisukela emakhazeni kuya ezifweni ezimbi kakhulu njenge-acute acute kupumua syndrome (SARS-CoV).I-SARS-CoV-2 uhlobo olusha olungakaze lutholakale kubantu ngaphambilini."Isifo seCoronavirus 2019" (COVID-19) sibangelwa igciwane elithi "SARS-COV-2" ukutheleleka.Iziguli zakwa-SARS-CoV-2 zibike izimpawu ezithambile (kuhlanganise neziguli ezingazange zibike izimpawu) zibe zimbi kakhulu.Izimpawu ze-COVID -19 zibonakala njengomkhuhlane, ukukhathala, ukukhwehlela okomile, ukuphelelwa umoya nezinye izimpawu, ezingakhula ngokushesha zibe yinyumoniya enzima, ukwehluleka ukuphefumula, ukushaqeka kwe-septic, ukwehluleka kwezitho eziningi, ukuphazamiseka kwe-acid-base metabolism, njll. Lokhu kungase kusongela ukuphila futhi kudingekile ngokushesha Yenza ukuhlolwa okusheshayo ukuze ulawule ubhadane lwamanje.

Ikhithi entsha ehlonza i-coronavirus IgM/IgG ye-antibody yakhelwe ukuthola ngokufanelekile amasosha omzimba we-SARS-CoV-2 futhi ayisebenzise njengethuluzi elisizayo ekuxilongeni ukutheleleka kwe-SARS-CoV-2.

Umgomo wokutholwa

Ikhithi iqukethe (1) inhlanganisela yomaka we-neocoronavirus antigen kanye nezimpawu zamaphrotheni zokulawula ikhwalithi kanye (2) nemigqa emibili yokutholwa (i-T1 ne-T2, ngokulandelanayo ehlanganiswe namasosha omzimba e-IgM kanye ne-IgG) kanye nomugqa wokulawula ikhwalithi (okuhlanganisa Ngokulawula ikhwalithi anti-protein antibody).Lapho isampula yengezwa emculweni wokuhlola, iphrotheni enelebula legolide le-SARS-CoV-2 izobopha amasosha omzimba e-IgM kanye/noma e-IgG akhona kusampula ukuze akhe i-antigen-antibody complex.Lezi zakhiwo zihamba emgqeni wokuhlola, bese zithwetshulwa i-anti-human antibody IgM kulayini we-T1, futhi/noma i-anti-human IgG antibody kulayini we-T2, kuvela ibhande elibomvu-nsomi endaweni yokuhlola, ebonisa umphumela omuhle.Uma lingekho i-anti-SRAS-CoV-2 antibody kusampula noma izinga le-antibody kusampula liphansi kakhulu, ngeke kube khona imigqa ebomvu-nsomi kokuthi “T1 kanye ne-T2″."Umugqa wokulawula ikhwalithi" usetshenziselwa ukulawula inqubo.Uma inqubo yokuhlola iqhubeka ngokujwayelekile futhi ama-reagents asebenza kahle, umugqa wokulawula ikhwalithi kufanele uhlale uvela.

Ama-reagents anikeziwe

Ikhithi ngayinye iqukethe:

Inqubo yokuthola

Funda leli bhukwana ngokucophelela ngaphambi kokusebenza ukuze ugweme imiphumela engalungile.

1. Ngaphambi kokuhlolwa, wonke ama-reagents kufanele alingane nezinga lokushisa legumbi (18 kuya ku-25°C).

2. Khipha ikhadi lokuhlola esikhwameni se-aluminium foil bese ulibeka endaweni eyisicaba, eyomile.

3. Isinyathelo sokuqala: Sebenzisa i-pipette noma udlulise i-pipette ukwengeza u-10μL we-serum/plasma, noma u-20μL wegazi eliphelele lomunwe noma igazi eliphelele le-venous emthonjeni wesampula.

4. Isinyathelo sesi-2: Ngokushesha engeza amaconsi angu-2 (60µL) esampula sebhafa kumthombo wesampula.

5. Isinyathelo sesi-3: Uma ukuhlolwa kuqala ukusebenza, ungabona umbala obomvu uhamba efasiteleni lokusabela phakathi nendawo yekhadi lokuhlola, futhi umphumela wokuhlola uzotholakala phakathi kwemizuzu engu-10-15..

Ukuhunyushwa kwemiphumela

Okuhle (+)

1. Kukhona imigqa ebomvu emi-3 (T1, T2, no-C) efasiteleni lokusabela.Noma ngabe yimuphi umugqa ovela kuqala, ukhombisa ukuba khona kwamasosha omzimba amasha e-coronavirus IgM kanye ne-IgG. 2. Kunemigqa emi-2 ebomvu (T1 no-C) efasiteleni lokusabela, kungakhathaliseki ukuthi yimuphi umugqa ovela kuqala, ubonisa ukuba khona kwamasosha omzimba e-coronavirus e-IgM amasha. 3. Kunemigqa emibili ebomvu (i-T2 no-C) efasiteleni lokusabela, kungakhathaliseki ukuthi yimuphi umugqa ovela kuqala, ubonisa ukuba khona kwamasosha omzimba e-coronavirus e-IgG.

Okubi(-)

1. Umugqa “C” kuphela (umugqa wokulawula ikhwalithi) efasiteleni lokusabela obonisa ukuthi awekho amasosha omzimba atholakala ku-coronavirus entsha, futhi umphumela uba negethivu.

Akuvumelekile

1. Uma ulayini wokulawula ikhwalithi (C) ungaboniswa phakathi kwemizuzu eyi-10-15, umphumela wokuhlola awuvumelekile kungakhathaliseki ukuthi kukhona umugqa we-T1 kanye/noma we-T2.Kunconywa ukuthi uphinde uhlole. 2. Umphumela wokuhlolwa awuvumelekile ngemva kwemizuzu eyi-15.

Ngakho-ke ungenza lokhu kuhlola ekhaya, uthumele i-imeyili noma ushayele ukuze uthole imininingwane eyengeziwe mayelana nekhithi yokuthola i-antibody ye-Sars-CoV-2 IgM/IgG (indlela yegolide ye-colloidal).