I-SARS-CoV-2 Nucleic Acid Detection Kit (I-Multiplex PCR Fluorescent Probe Method)
Ukusetshenziswa Okuhlosiwe
Isimiso sokuhlola
Lesi siqalisi kanye namasethi ophenyo aklanyelwe ukutholwa kokulandelana okulondoloziwe kwe-SARS-CoV-2 (ORF1ab gene, N gene and E gene).Ikhithi iqukethe Ukulawulwa Kwangaphakathi okusetshenziselwa ukuhlola ikhwalithi yesifanekiso.Ubuchwepheshe be-PCR besikhathi sangempela busebenzisa i-polymerase chain reaction (PCR) ukuze kukhuliswe ukulandelana okuqondiwe okuqondile kanye nama-probe aqondiwe ukuze kutholwe i-RNA ekhulisiwe.Ama-probe abhalwe nge-fluorescent reporter kanye nodayi bokucisha.
Imininingwane Esheshayo
◮Imininingwane:48 Rxns/kit, 96 Rxns/kit
◮ Ukucaciswa:96.72%
Ukuzwela:97.92%
Izingxenye
Cha. | Isakhi | Inani | Isithako esiyinhloko | |||
awu 48r | awu 96r | |||||
1 | Ukukhishwa kwe-Nucleic acidumenzeli | 1.5 mL / ishubhu | 1 ithubhu | 1.5 mL / ishubhu | 1 ithubhu | I-surfactant |
2 | Isivikeli se-RNA | 27 μL/ithubhu | 1 ithubhu | 53 μL/ithubhu | 1 ithubhu | I-RNase inhibitor |
3 | I-SARS-CoV-2isixazululo sokusabela | 800 μL / ishubhu | 1 ithubhu | 1600 μL/ipayipi | 1 ithubhu | I-Primer, probe, buffer yokusabela, i-dNTP |
4 | I-SARS-CoV-2inhlanganisela ye-enzyme | 80 μL/ithubhu | 1 ithubhu | 160 μL/ishubhu | 1 ithubhu | Isiqalo esishisayo se-Taq enzyme,I-M-MLV enzyme |
5 | I-SARS-CoV-2ukulawula okuhle | 100 μL / ishubhu | 1 ithubhu | 100 μL / ishubhu | 1 ithubhu | I-Recombinant plasmid equkethe ithagethiingxenye, i-RNA |
6 | I-SARS-CoV-2ukulawula okungalungile | 1200 μL / ishubhu | 1 ithubhu | 1200 μL / ishubhu | 1 ithubhu | Ibhafa ye-TE |
Izici&izinzuzo
Isetshenziswa kanjani
Indlela yoku-1: Indlela ye-PCR eqondile
Indlela 2: I-Viral RNA Isolation + PCR
Indlela 1: Indlela ye-PCR eqondile usebenzisaUmenzeli Wokukhishwa Kwesampula
I-Cold Chain Ezokuthutha kanye Nesitoreji
Ivalwe ekukhanyeni futhi igcinwe ku -20±5℃;
Impilo yeshelufu
1 unyaka