I-RT-qPCR Easyᵀᴹ (Isinyathelo esisodwa)-SYBR Green I
Incazelo yekhithi:
◮Ikhithi yesinyathelo esisodwa yenza ukuloba okuhlanekezelwe kanye ne-qPCR ukusabela okubili kushubhu efanayo, idinga kuphela ukwengeza ithempulethi i-RNA, iziqalo ezithile ze-PCR kanye ne-RNase-Free ddH.2O.
◮Ikhithi ingahlaziya ngokushesha nangempumelelo i-RNA yegciwane noma ilandele i-RNA.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
◮I-RT-qPCR EasyTM(One Step) -SYBR Green I kit iza nodayi wereferensi wangaphakathi we-ROX, ongasetshenziswa ukuqeda ingemuva lesignali namaphutha esignali phakathi kwemithombo, okulula ukuthi amakhasimende ayisebenzise kumamodeli ahlukene wamathuluzi e-PCR amaningi.
Izincazelo
I-RT-qPCR EasyTM (Isinyathelo esisodwa)-SYBR Green I isebenzisa isistimu eyingqayizivele ukuhlanganisa ngempumelelo ukusabela kwe-RT kanye Nesikhathi Sangempela se-PCR, engaqedela ngokushesha ukutholwa kobuningi bezakhi zofuzo.Umkhiqizo unokubekezelelana okuqinile, ukucaciswa okuphezulu nokuzinza.Umkhiqizo uqukethe i-Foregene HotStar Taq DNA Polymerase yenkampani eyingqayizivele, enezinzuzo zokukhulisa ngokushesha (2Kb/min), ukusebenza kahle kokukhulisa ukuphakama, ikhono eliqinile lokukhuliswa okukhethekile, nezinga lokungafani eliphansi uma liqhathaniswa nama-enzyme e-Taq ajwayelekile.Isetshenziswa lapha ku-Real Time RT-PCR ukuze kwehliswe ukukhulisa okungaqondile nokuthuthukisa ukunemba kwe-PCR.
Imininingwane
100 T (20μl uhlelo)
500 T (20μl uhlelo)
Izingxenye zekhithi
| I-RT-qPCR EasyTM(Isinyathelo esisodwa)-SYBR Green I | ||
| Okuqukethwe Kwekhithi | RT-02111 | RT-02112 |
| 100T (20μl uhlelo) | 500T (20μl uhlelo) | |
| 2× RT-qPCR EasyTM I-Mix-SYBR | 1ml | 1.7ml×3 |
| I-50 × I-ROX Reference Dye | 200μl | 1ml |
| I-ddH yamahhala ye-RNase2O | 1.7ml | 1.7ml×3 |
| Incwadi Yeziqondiso | 1 ucezu | 1 isiqephu |
Izici&izinzuzo
- Ikhithi yesinyathelo esisodwa yenza ukuloba okuhlanekezelwe kanye ne-qPCR ukusabela okubili kushubhu efanayo, idinga kuphela ukwengeza ithempulethi i-RNA, iziqalo ezithile ze-PCR kanye ne-RNase-Free ddH2O.
- Ikhithi ingahlaziya ngokushesha nangempumelelo i-RNA yegciwane noma ilandele i-RNA.
- Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
- Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela okuphezulu kokutholwa, ukuqina okushisayo okuqinile, nokubekezelelana okungcono.
- RT-qPCR EasyTM(One Step) -SYBR Green I kit iza nodayi wereferensi wangaphakathi we-ROX, ongasetshenziswa ukuqeda ingemuva lesignali namaphutha esignali phakathi kwemithombo, okulula ukuthi amakhasimende ayisebenzise kumamodeli ahlukene wamathuluzi e-PCR amaningi.
Ukuhamba komsebenzi
Izimo zokugcina
1.Ezokuthuthaizimo
Yonke inqubo yokuthutha kwebhokisi leqhwa elinezinga lokushisa eliphansi, ukuqinisekisa ukuthi ikhithi isesimweni esingu-<4 ℃.
2.Isitoreji izimo
Ikhithi igcinwa ku -20 ℃.Gcina umkhiqizo efrijini lokushisa njalo at-20 ℃ ngokushesha ngemva kokuthola.Uma izimo zokugcina zifanelekile, umkhiqizongeke kwehlise isithunzi sanoma yikuphi ukusebenza phakathi nesikhathi sokuqinisekisa sonyaka ongu-1.
