SAWONA, bangane!

Ngemva kokwenza i-nucleic acid extraction iminyaka eminingi kangaka, wonke umuntu uyazi kahle ukuthi izinga le-nucleic acid extraction liyisihluthulelo sempumelelo nokunemba kokuhlolwa okulandelayo.

Izikhathi eziningi, sinendaba nokuthi isilinganiso se-OD260/OD280 singaphakathi kwebanga elifanele.Kanje nje...

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Njengoba isisho sisho, awukwazi ukukhuluma kakhulu, futhi awukwazi ukujabula kusenesikhathi.

Qala ulalele Mhleli ekuxoxela indaba encane!

Izinganekwane ezinjalo ezinhle zivame ukwenzeka kumalabhorethri akhipha i-nucleic acid.

Ubucabanga ukuthi uthathe i-RNA kuphela, Uxolo!

Eqinisweni, yingoba awazi ukuthi uMnu Protein ucasha ngasese.

Ingabe ucabanga ukuthi ufuna ukwenza uMnu Protein abonakale nge-OD260/OD280 eyodwa kuphela?

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Mncane kakhulu!Kulula kakhulu!

Masisebenzise indlela ebucayi kakhulu yokwenza amabala esiliva ukukusiza ubone amaprotheni afihlwe ku-RNA!

Umkhiqizo osetshenzisiwe

OD260/280

Minsimbi yokulinganisa

I-Foregene

2.09

I-Thermo ScientificTMI-NanoDropTMEyodwa

I-Ultra-trace UV-Vis Spectrophotometer

I-Trizol

1.91

Kudala, amaprotheni ne-RNA kwakungabangane abakhulu.

UMnu Protein unamahloni kakhulu.
Ngakho bekuhlezi kuyi-RNA esebenzayo nejabulisayo ukudlala ngayo.

Usuku olulodwa.
I-RNA yatshela uMnu Protein ukuthi izothathwa abanye, futhi uMnu Protein wayedabukile kakhulu.

I-RNA iphinde ibe manqikanqika kakhulu kuMnuz protein, futhi yathi: "Kungenzeka kanjani ukuthi ngikuthumbe, ucashe ezingutsheni zami, uzame ukuthi ungabonwa ngabanye, kulungile?"

UMnu. Protein wagqabuka emamatheka futhi wavuma ngenjabulo.

Ngosuku lokutholwa, uMnu Protein wacasha ngempela ezingutsheni ze-RNA futhi akatholakalanga.Walandela umuntu wokutholwa ekhaya futhi waphila impilo ejabulisayo kusukela ngaleso sikhathi.

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Ukuqhathanisa ukukhishwa kwe-RNA no-ForegeneIseliIsambaIkhithi yokukhipha i-RNA

kanye ne-T Company's Trizol Reagent

Singabona ngokucacile ibhande kumzila 4 (Trizol), kuyilapho umzila 3 (Forgene) uhlanzekile uma kuqhathaniswa.Imiphumela yaqinisekisa ukuthi ukungcola kwamaprotheni e-RNA akhishwa ngendlela ye-Trizol kwakusengamaconsi amaningi!Futhi umzila 3 (Forgene) cishe awunakho ukungcola kwamaprotheni ngoba umhloli usebenzise ikhithi yokukhipha i-Foregene RNA!(Isikhangisi sifike ngingalindele)

Ulwazi olwengeziwe:

https://www.foreivd.com/cell-total-rna-isolation-kit-product/

https://www.foreivd.com/reagent/rna-isolation-series/


Isikhathi sokuthumela: Feb-10-2022