SAWONA, bangane!
Ngemva kokwenza i-nucleic acid extraction iminyaka eminingi kangaka, wonke umuntu uyazi kahle ukuthi izinga le-nucleic acid extraction liyisihluthulelo sempumelelo nokunemba kokuhlolwa okulandelayo.
Izikhathi eziningi, sinendaba nokuthi isilinganiso se-OD260/OD280 singaphakathi kwebanga elifanele.Kanje nje...
Njengoba isisho sisho, awukwazi ukukhuluma kakhulu, futhi awukwazi ukujabula kusenesikhathi.
Qala ulalele Mhleli ekuxoxela indaba encane!
Izinganekwane ezinjalo ezinhle zivame ukwenzeka kumalabhorethri akhipha i-nucleic acid.
Ubucabanga ukuthi uthathe i-RNA kuphela, Uxolo!
Eqinisweni, nje ukuthi awazi ukuthi uMnu Protein ucasha ngasese.
Ingabe ucabanga ukuthi ufuna ukwenza uMnu Protein avele nge-OD260/OD280 eyodwa kuphela?
Mncane kakhulu!Kulula kakhulu!
Masisebenzise indlela ebucayi kakhulu yokwenza amabala esiliva ukukusiza ubone amaprotheni afihlwe ku-RNA!
Umkhiqizo osetshenzisiwe | OD260/280 | Minsimbi yokulinganisa |
I-Foregene | 2.09 | I-Thermo ScientificTMI-NanoDropTMEyodwa I-Ultra-trace UV-Vis Spectrophotometer |
I-Trizol | 1.91 |
Kudala, amaprotheni ne-RNA kwakungabangane abakhulu.
UMnu Protein unamahloni kakhulu.
Ngakho bekuhlale kuyi-RNA esebenzayo nejabulisayo ukudlala ngayo.
Usuku olulodwa.
I-RNA yatshela uMnu Protein ukuthi izothathwa abanye, futhi uMnu Protein wayedabukisa kakhulu.
I-RNA iphinde ibe manqikanqika kuMnuz protein, futhi yathi: "Kungenzeka kanjani ukuthi ngikuntshontshe, ucashe ezingutsheni zami, uzame ukuthi ungabonwa ngabanye, kulungile?"
UMnu. Protein wagqabuka emamatheka futhi wavuma ngenjabulo.
Ngosuku lokutholwa, uMnu Protein wacasha ngempela ezingutsheni ze-RNA futhi akatholakalanga.Walandela umuntu wokutholwa ekhaya futhi waphila impilo ejabulisayo kusukela ngaleso sikhathi.
Ukuqhathanisa ukukhishwa kwe-RNA no-ForegeneIseliIngqikithiIkhithi yokukhipha i-RNA
kanye ne-T Company's Trizol Reagent
Singabona ngokucacile ibhande kumzila 4 (Trizol), kuyilapho umzila 3 (Forgene) uhlanzekile uma kuqhathaniswa.Imiphumela yaqinisekisa ukuthi ukungcola kwamaprotheni e-RNA akhishwa ngendlela ye-Trizol kwakusengamaconsi amaningi!Futhi umzila 3 (Forgene) cishe awunakho ukungcola kwamaprotheni ngoba umhloli usebenzise ikhithi yokukhipha i-Foregene RNA!(Isikhangiso sifike ngingalindele)
Ulwazi olwengeziwe:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Isikhathi sokuthumela: Feb-10-2022