Izindlela ze-PCR kanye nokungcoliswa kwe-aerosol ye-nucleic acid kumalabhoratri okuhlola i-nucleic acid kufana nezinhlangothi ezimbili zohlamvu lwemali.Singakhetha kuphela ukuba nayo noma cha, kodwa ngeke sikhethe ukuthi siyayifuna noma siyayisebenzisa.

1. Ukuhlolwa kwesikhiphi se-DNA

Ukuze kuzuzwe ukususwa kwendawo yokungcoliswa kwe-aerosol ye-nucleic acid, kuyadingeka kuqala ukuthi kuhlolwe izikhiphi ze-DNA ezingasusa ama-nucleic acid esimweni samanzi.Ngoba azikho eziningi zokususa i-DNA ezisebenza ngempela.Ukuze uthole indlela yokuhlola, sicela ubheke: Isikhiphi se-DNA asikwazi ukuba "ikhona eliyimfihlo" lelabhorethri!

Kulesi sivivinyo, amakhophi angu-100/μL (CT cishe angama-31) e-ASFV plasmid kanye nesikhiphi se-DNA esilinganiswa nge-PCR yedijithali axutshwe ngamavolumu alinganayo, abese esabela kuzinga lokushisa legumbi i-10min, 20min kanye ne-30min ngokulandelanayo.Ngemuva kokukhipha i-nucleic acid, ukukhulisa i-qPCR kwenziwa.Ukulawula okuhle okuxutshwe ne-plasmid namanzi kwaqhathaniswa.Njengoba ukuhlolwa kungazange kuqedwe ngesikhathi esifanayo, kungase kube khona ukuchezuka okuthile phakathi kwemiphumela, kodwa akuthinti isiphetho sokuhlolwa.Kuze kube manje, ngihlole imikhiqizo eyi-10 ye-DNA remover.Imikhiqizo engu-No. 1, No. 6 kanye No. 8 kuphela engakwazi ukonakalisa i-plasmid DNA esimweni samanzi.Eminye imikhiqizo cishe ayinawo umphumela.

Ithebula 1 Umthelela wokususwa kwe-Nucleic acid yesikhiphi se-DNA esitholakalayo esithengiswayo

2. Ukuhlolwa komphumela wokususwa kwe-DNA kwezibulala-magciwane ezisetshenziswa kakhulu

1. Ukuhlolwa kwezibulala-magciwane ezisetshenziswa kakhulu

Kunezinhlobo eziningi zezibulala-magciwane ezivame ukusetshenziswa ezindaweni zokucwaninga: ama-aldehydes, ama-phenol, ama-alcohol, ama-quaternary ammonium salts, ama-peroxides, amalungiselelo e-chlorine, nama-asidi nezisekelo.Imiphumela yokubulala amagciwane yalezi zibulali magciwane kuma-microorganisms iqinisekiswe ngokugcwele ngokuhlolwa, kodwa Ayikho idatha eyanele yokuhlola ngomphumela wokuwohloka kwama-nucleic acid.Amalabhorethri okuhlola i-Nucleic acid adinga isibulala-magciwane esingagcini nje ngokuba nomthelela omuhle wokubulala amagciwane kuma-microorganisms, kodwa futhi esingehlisa isithunzi i-DNA.Imiphumela yokuhlolwa ikhombisa ukuthi amalungiselelo e-chlorine amabili kuphela e-84 isibulala-magciwane kanye ne-trichloroisocyanuric acid ne-1 M hydrochloric acid ahlangabezana nezidingo.Njengoba i-1 M hydrochloric acid igqwala kakhulu, kunconywa ukuthi amalungiselelo e-chlorine asetshenziswe njengokulahlwa kukadoti kumalabhorethri okuhlola i-nucleic acid.Kodwa-ke, amalungiselelo e-chlorine ayagqwala ezinsimbi futhi awakwazi ukusetshenziselwa ukubulala amagciwane amathuluzi nezinto zokusebenza.

Ithebula 2 imiphumela yokususwa kwe-DNA yezinhlobo ezahlukene zezibulala-magciwane

2. Isilinganiso esincane esisebenzayo samalungiselelo e-chlorine

Izibulala-magciwane ezisekelwe ku-chlorine zinomphumela onamandla wokuwohloka kwe-DNA, kodwa ngenxa yokugqwala kwazo kwensimbi nokucasuka, zingasetshenziswa ukuhlanza phansi, ama-countertops angewona awensimbi, amathiphu okucwiliswa, amashubhu e-centrifuge nezinye izinto zokuhlola.

Ngokwe "New Coronary Pneumonia Epidemic Disinfection Technical Guidelines": Izinto ezingcolisayo (igazi leziguli, uketshezi kanye nokuhlanza) i-chlorine etholakalayo engu-5g/L-10g/L ene-chlorine equkethe isibulala-magciwane;phansi, izindonga, nezindawo zisebenzisa i-chlorine etholakalayo engu-1g/L Isibulala-magciwane Se-chlorine: izingubo, izingubo zokulala nezinye izindwangu kuqala kucwiliswe kusibulala-magciwane se-chlorine nge-chlorine engu-0.5g/L etholakalayo imizuzu engu-30, bese kuhlanzwa njengenjwayelo.

Ithebula 3 Umphumela wokususwa kwe-DNA yokugxila okuhlukene kwesibulala-magciwane se-chlorine

Imiphumela yokuhlola ibonisa ukuthi: lapho ukugxiliswa kwe-chlorine okusebenzayo kukhulu noma kulingana no-1.2 g/L, isibulala-magciwane esine-chlorine singehlisa isithunzi ngokuphelele amakhophi ayi-100/μL we-plasmid uma sisetshenziswa imizuzu emi-5.Lapho usebenza imizuzu eyi-10, isibulala-magciwane esingu-84 esine-chlorine eningi noma elingana no-0.6 g/L singehlisa isithunzi ngokuphelele amakhophi ayi-100/μL ama-plasmids.

3. Ukuhlolwa kokukhipha umoya okungcoliswe i-nucleic acid aerosol

Indlela yokuhlanza umoya ongcoliswe ama-nucleic acid aerosols?Iningi lemicabango iwukwenza indawo engcoliswe i-aerosol bese uyihlanza, noma ukuhlanza ama-nucleic acid ngemva kokungcola.Kimi, ukuhlola okungenakuphindwa futhi imiphumela yokuhlolwa ayikwazi ukuhlaziywa ngokwesilinganiso ayinangqondo, ngakho-ke ngiboleke ezinye izindlela zokubulala amagciwane emoyeni.Bona "Izimfuneko Ezijwayelekile Ze-GB27948-2020 Zokubulala Amagciwane Omoya" kanye "Nezicaciso Zobuchwepheshe Zokubulala Amagciwane"

1. Izinto zokuhlola

1.1 Isikhiphi se-DNA: Kulesi sivivinyo, izikhiqizi ze-DNA ezimbili ezitholakalayo ezithengiswayo 6 no-8 kuThebula 1 zikhethiwe.

1.2 I-plasmid yokuhlola: Uma kucatshangelwa ukuthi inani le-CT lokungcoliswa kwelabhorethri okuningi lizoba phezulu kunokungu-30, ukugcwala kwe-plasmid yofuzo ye-ASFV esetshenziswe ngalesi sikhathi cishe amakhophi angu-100/μL, kanye ne-fluorescent quantitative PCR CT icishe ibe ngu-31.07.Engeza i-plasmid ku-swab kakotini egelezayo futhi wome emoyeni.

1.3 Ithuluzi lokuhlola: Ithuluzi lokubulala amagciwane i-Blingkehan KVBOX, elisekelwa ngomusa i-Shenzhen Runlian Environmental Technology Co., Ltd., linokusebenza okuhle kakhulu kokubulala amagciwane ngesibulala-magciwane se-hydrogen peroxide, futhi lingabulala ngempumelelo izinhlamvu nezinye izinto ezincane.

1.4 Indawo yokuhlola: iwindi lokudlulisa elivaliwe elingama-cubic metres angu-0.1.

2. Indlela yokuhlola

Inani lesibulala-magciwane esisethiwe emshinini wokukhipha amagciwane ngu-10ml, futhi ukugxiliswa ku-100ml/m3, ephakeme kakhulu kune- "GB27948-2020 General Requirements for Air Disinfectants": lapho usebenzisa isibulala-magciwane se-aerosol, inani lesibulala-magciwane kufanele libe ≤10 ml/m3