Iyini i-DirectPCR?
Ubuchwepheshe be-DirectPCR busho ukuthi ngaphandle kokuhlukanisa nokuhlanza ama-nucleic acid ama-nucleic (okuhlanganisa i-DNA ne-RNA) yamasampula ezicubu ezihlukahlukene, izicubu kanye nesakhiwo seseli kuphela esibhujiswa yi-lysis ye-protease, i-nucleic acid ikhishwa kusisombululo se-lysis, futhi isisombululo se-lysis sengezwe ngokuqondile.I-PCR reaction system iwubuchwepheshe bokukhulisa isakhi sofuzo esiqondiwe.
Ufuna ushintsho
Ukuhlukaniswa nokukhishwa kwe-nucleic acid udaba
Kusukela kwasungulwa ubuchwepheshe be-PCR eminyakeni engu-30 edlule, abacwaningi baye bakhathazwa ukuhlukaniswa nokukhishwa kwama-nucleic acid.Ukusebenzisa amasampula ezicubu ukwenza ngokuqondile ukusabela kwe-PCR kuyiphupho labacwaningi abaningi.Kodwa leli phupho alizange ligcwaliseke iminyaka engu-30.Isizathu siwukuthi izicubu ezihlakazekile zizokhipha izithako eziningi ezivimbelayo.
Lezi zingxenye ezivimbelayo zizoba nomphumela oqinile wokuvimbela ekuphenduleni kwe-PCR, ezinye zizobangela ukuthi i-primer kanye nesifanekiso singakwazi ukubopha, ezinye zibe nomsebenzi oqinile we-protein denaturation, okuholela ekungasebenzini kwe-nucleic acid polymerase, kanti ezinye zivimbela ngokuqondile i-primer nesifanekiso ekubopheni .Lezi zonke izici ezibangela ukuthi ukusabela kwe-PCR kungaqhubeki kahle.
Ngokwesiko, abantu ngezikhathi ezithile basebenzisa izithuthukisi ze-PCR ukuze bathuthukise ukuzwela, ukucacisa kanye nokusebenza kahle kokukhulisa ukusabela kwe-PCR.Kodwa-ke, ngenxa ye-PCR inhibitor ehlukene equkethwe kuzifanekiso ze-nucleic acid ezivela emithonjeni ehlukene, ukusetshenziswa kwesikhathi eside kwenani elikhulu lezithuthukisi ngokusobala akufanele ukulahlekelwa, izindleko ziphezulu, futhi ukusebenza kunzima.
I-Foregene DirectPCR
Ukuphumelela okuhamba phambili emhlabeni --Amasu Amabili
U-Foregene wenze impumelelo kulo mkhakha wezobuchwepheshe emhlabeni jikelele.Ubuchwepheshe be-Foregene DirectPCR bunamaphuzu amabili obuchwepheshe, okwenza i-Foregene ibe iphayona nomholi wobuchwepheshe be-DirectPCR.
Okokuqala, indlela yokuguqulwa kwe-nucleic acid polymerase enelungelo lobunikazi.I-Forgene inendlela yokuguqula i-nuclease enelungelo lobunikazi, eqondiswe esizindeni esibophezelayo ukuze yenze i-nuclease nesifanekiso kuqine, ngomsebenzi ophakeme, ukusebenza kahle kokukhulisa izinga eliphezulu nokuzinza kokushisa.
Okwesibili, ifomula ye-PCR Mix elungiselelwe izinhlobo ezihlukene, izithuthukisi zokusabela eziyingqayizivele, izilungiseleli kanye neziqinisi, ithuthukisa kakhulu ukumelana kwe-polymerase kuma-PCR inhibitors futhi iqinisekisa ukusebenza kahle kokukhulisa.
Kungenxa yokuphumelela okukhulu okukhulunywe ngenhla kwezobuchwepheshe okwenza i-Foregene ithole i-DirectPCR yangempela emhlabeni jikelele.Kungakhathaliseki ukuthi izicubu zezilwane ezivamile, uketshezi lomzimba, izicubu zezitshalo, amaqabunga noma amathiphu ezimpande, noma ngisho nembewu yezitshalo, abasebenzisi bangakwazi ukufinyelela kalula ukukhuliswa kwe-PCR okuqondile ngaphandle kokuphuka kwemishini noma inqubo yokuhlanza i-nucleic acid eyisicefe.
Okwamakhithi e-DirectPCR ochungechunge lwezitshalo kanye nochungechunge lwezilwane oseluqalisiwe, i-Foregene ingasho ngokuziqhenya ukuthi sinezinkomba zokusebenza ezihamba phambili emhlabeni.Ngokuzayo, i-Foregene izoqhubeka nokwethula uchungechunge lwemikhiqizo esenezinkomba zokusebenza ezihamba phambili emhlabeni (noma ngisho eyingqayizivele).
Ukuze uthole ulwazi olwengeziwe, faka:
http://www.foregene.com/http://www.foreivd.com/
Isikhathi sokuthumela: Feb-27-2017