W:Mfowethu, ukubhala okuphambene kuqediwe, futhi ekugcineni isinyathelo sokugcina - i-fluorescent quantitative PCR!Sawubona, mfowethu, yini i-PCR yesikhathi sangempela?
M:PCR uhlale uyazi, akunjalo?
W:Uyazi, i-PCR iyi-polymerase chain reaction, futhi iwubuchwepheshe obandisa i-DNA!
M:Kucishe kube… Kuyindlela yobuchwepheshe yokukhulisa ukulandelana okuqondiwe kuye izikhathi ezingaphezu kwesigidi ngokusebenzisa ama-primers athile, kodwa lokhu kubonisa kuphela ukuba khona noma ukungabikho kokulandelana okuqondiwe, futhi akukwazi ukunquma inani lesifanekiso sokuqala.I-Fluorescence quantitative PCR iyindlela yobuchwepheshe eyakhelwe ukuxazulula le nkinga.I-PCR yesikhathi sangempela kanye ne-quantity PCR (qPCR) ngokuvamile ibhekisela kuyo.
W: Umehluko ovela ku-PCR evamile usekulinganisweni kwe-fluorescence?
M:Yebo, okuwukuthi, esigabeni sokunwetshwa somjikelezo we-PCR ngamunye, kutholwa ukuqina kwesiginali ye-fluorescence, futhi inani lesifanekiso sokuqala singabalwa ngokuya ngokushintsha kokuqina kwesiginali.
W: Kuzwakala kuyinkimbinkimbi kakhulu!Ingabe kufanele ngibale lokhu ngedwa?
M: Lokhu kudinga ukuthi ufunde kancane, kungcono ukwazi ukuqonda isimiso.Manje usungakwazi ukugxila enanini le-CT kuqala, okuyidatha yokuhlola enembile, futhi usayizi wayo uhambisana nosayizi wenani lesifanekiso sokuqala.Kukhona futhi amagrafu afana namajika okukhulisa i-amplification namajika ancibilikayo.Banezwi lokugcina lokuthi ukuhlola kwakho kuphumelele noma cha.
W: O, mfowethu.Bese ngizokwenza isilingo se-qPCR!
M:Naka ukunaka okukhethekile ukuthi ungangezi izifanekiso endaweni ye-reagent, ngaphandle kwalokho imiksi yakho engasetshenzisiwe izongcoliswa kalula!
W: Ngiyezwa!

Isikhathi Sangempela PCR Easyᵀᴹ-SYBR Green I Kit

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