Isici esivelele sokusabela kwe-PCR amandla ayo amakhulu okukhulisa nokuzwela okuphezulu kakhulu.Ukuze kuthuthukiswe ukusebenza kwe-PCR nokusebenza kahle kokutholwa, sizibophezele ekuthuthukiseni umthamo wokukhulisa i-PCR nokuzwela kokutholwa, kodwa ikhanda elibuhlungu lisenqubweni yokuhlola.Okuhle okungamanga kuvame ukwenzeka, futhi inani elincane kakhulu lesampula lokungcoliswa okuphambene noma ukungcoliswa komkhiqizo we-PCR kungase kubangele imibono engamanga ekuhlolweni.
Izinhlobo ezinhlanu zokungcoliswa komkhiqizo we-PCR
Kunezizathu eziningi zokungcola kwe-PCR, ezingahlukaniswa cishe zibe yizigaba ezilandelayo:
Ukungcoliswa kwe-specimen kubangelwa ikakhulukazi ukungcoliswa kwesiqukathi sokuqoqa isampula, noma uma isifanekiso sibekwe, siphuma ngaphandle kwesitsha ngenxa yokuvaleka okuxekethile, noma isifanekiso sinamathele ngaphandle kwesiqukathi, okubangela ukungcoliswa okuphambene;Ukungcola kuholela ekungcoleni phakathi kwezibonelo;ezinye izibonelo zama-microbial, ikakhulukazi amagciwane, angasabalala ngama-aerosol noma akhe ama-aerosol, okuholela ekungcoleni okufanayo.
Isizathu esiyinhloko ukuthi ngesikhathi sokulungiswa kwama-PCR reagents, isampula yesibhamu, isitsha, amanzi acwecwe kabili nezinye izixazululo zingcoliswe isifanekiso se-PCR nucleic acid.
Kulabhorethri yebhayoloji yamangqamuzana nakwamanye amalabhorethri asebenzisa ama-plasmid ahlanganisiwe njengezilawuli ezinhle, inkinga yokungcoliswa okuhlanganisiwe kwe-plasmid nayo ivamile.Ngenxa yokuthi okuqukethwe kwe-cloning plasmid kumthamo weyunithi kuphezulu kakhulu, futhi amathuluzi amaningi nama-reagents ayadingeka enqubweni yokuhlanza, futhi i-plasmid emangqamuzaneni aphilayo kungenzeka ukuthi angcoliswe ngenxa yokukhula okuqinile nekhono lokuzala lamangqamuzana aphilayo.
Ukungcoliswa kwemikhiqizo ekhulisiwe kuyinkinga yokungcola evame kakhulu ekuphenduleni kwe-PCR.Ngenxa yokuthi inani lekhophi lomkhiqizo we-PCR likhulu (ngokuvamile amakhophi angu-1013/ml), eliphakeme kakhulu kunomkhawulo wenombolo yekhophi yokutholwa kwe-PCR, inani elincane kakhulu lokungcoliswa komkhiqizo we-PCR lingabangela amanga.
Ukungcoliswa kwe-aerosol kuyindlela engenzeka kakhulu yokungcoliswa kwemikhiqizo ye-PCR, futhi futhi kulula kakhulu ukunganakwa.Yakhiwa ukungqubuzana phakathi kwendawo ewuketshezi nomoya.Ngokuvamile, ukungcoliswa kwe-aerosol kungakheka lapho kuvulwa isivalo, lapho isampula sishiswa, noma ngisho nalapho ishubhu lokusabela linyakaziswa ngamandla.Ngokwezibalo, ingxenyana ye-aerosol ingaba namakhophi angu-48,000, ngakho ukungcola okubangelwa yikho kuyinkinga edinga ukunakwa okukhethekile.
Ikakhulukazi, amalabhorethri okuhlola ngokuvamile asebenzisa ipheya efanayo yeziqalo ukuhlola isakhi sofuzo esithile.Ngokuhamba kwesikhathi, inani elikhulu lokungcoliswa komkhiqizo we-PCR lizokwenzeka endaweni yaselabhorethri.Uma ukungcola okunjalo sekwenzekile, kuba nzima ukukuqeda ngesikhathi esifushane.
Ezinhlotsheni ezintathu zokuqala zokungcola, singasebenzisa izindlela eziphumelelayo zokugwema, kodwa ukungcola okubangelwa imikhiqizo ye-PCR kunzima ukukuvimbela, ikakhulukazi ekwakhiweni kwama-laboratories e-PCR angajwayelekile.Enqubweni ye-PCR, lapho ithiphu ye-pipette imunca futhi ishaya uketshezi, futhi kuvulwa ikhava yeshubhu ye-PCR, kuzokwakhiwa i-aerosol.Amangqamuzana e-DNA athwala i-aerosol (i-aerosol eyodwa ingathwala amashumi ezinkulungwane ze-DNA) kunzima ukuwaqeda ngoba antanta emoyeni.Uma umjikelezo olandelayo wokuhlolwa kwe-PCR wethulwa, imiphumela engelona iqiniso izokwenzeka nakanjani.
Njengoba kuboniswe esithombeni esingezansi, ukulawula okunegethivu kuphinde kwakhulisa ibhendi yentshisekelo ehambisanayo:
Ingxenye yokuqala yalolu daba lokungcoliswa kwe-PCR nokuvimbela yethulwa lapha.Umagazini olandelayo uzokulethela ingxenye yesibili ethi "Ukuvimbela ukungcoliswa komkhiqizo we-PCR", ngakho hlala ubukele!
Isikhathi sokuthumela: Jul-25-2017
