Ukusungulwa kwenjini yesitimu kulethe uguquko lwezimboni, ukuthuthuka kwamakhompiyutha kwadala umhlaba wamanje wedijithali, futhi uguquko lwezobuchwepheshe ngokuqinisekile luzoletha inqubekelaphambili yezikhathi!Ukuzalwa kukaI-Cell Direct RT-qPCR KitI-Foregene isizoletha inkathi entsha yokutholwa kwamangqamuzana!

7min Thola isifanekiso se-RNA

Yebo, ukuzwe kahle, ungangabazi, ukusuka kuseli ukuya ku-RT-qPCR, manje izinyathelo ezimbili kuphela

Misa Ukusabela

Q:Ingabe ukukhipha i-RNA kwanele ngesivinini esinjalo?Ingabe kuzoba nokungcoliswa kwe-DNA?

A: Ungakhathazeki, imikhiqizo yethu iqhathaniswe inqwaba nokuqinisekiswa, futhi akukho mehluko uma kuqhathaniswa nemiphumela yokuhlola okuvamile kwe-RT-qPCR.Ngaphezu kwalokho, usayizi wesampula mncane, futhi ukuhlolwa kungenziwa okungenani ngamaseli akhulisiwe ayi-10.Kungakhathaliseki ukuthi ipuleti lomthombo we-6, 12, 24 noma 96, amangqamuzana angalaliswa ngokuqondile emithonjeni yepuleti, elula futhi esheshayo.Nge-5× Direct RT Mix kanye ne-2× Direct qPCR Mix emkhiqizweni, ithempulethi ye-RNA ingasetshenziselwa ukuhlehlisa ukusebenza kahle okuphezulu nokukhulisa okuthile, ngokuzwela okuphezulu, ukucaciswa okuqinile nokuzinza okuhle.Ikhithi ye-reverse transcriptase qPCR ilula kakhulu.

I-Cell Direct RT-qPCR Kitayinayo kuphela isistimu ye-lysis ephakeme kakhulu, kodwa futhi inokumelana okuqinile kwe-RT-qPCR reaction inhibitors, okwenza yonke ikhithi ibe nomkhawulo wokubona ophansi kakhulu, futhi isengathola umphumela omuhle kumaseli angu-10. Lesi sinyathelo esisodwa se-RT qPCR kit silula kakhulu.

Manje sekunezindlela ze-SYBR qPCR eziluhlaza kanye ne-Taqman qPCR ezitholakalayo.