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Esigabeni sokuqala sokuqubuka kwalesi sifo, ngenxa yentuthuko esheshayo, ukutholakala ngokushesha kweziguli okusolwa ukuthi kuyisihluthulelo sokuvimbela i-COVID-19.Amanye ama-reagents okuthola i-nucleic acid agunyaziwe anesikhathi esifushane sokuthuthukiswa, futhi kunezinkinga ezifana nokuqinisekiswa kokusebenza okusheshayo, ukuthuthukiswa kwe-reagent okunganele, nomehluko omkhulu phakathi kwamaqoqo;Izinkinga zamalabhorethri emtholampilo ahlukahlukene ezicini ezahlukahlukene zenqubo yokutholwa kwe-nucleic acid zingase futhi zithinte ukunemba kwemiphumela yokutholwa kwe-nucleic acid.Lesi sihloko sizogxila kuzixhumanisi ezibalulekile namaphuzu ekutholweni kwamanje kwe-nucleic acid yakwa-SARS-CoV-2, futhi sihlaziye izinkinga zokuhlolwa kabusha okungamanga okungekuhle nokuhle kokutholwa kwe-nucleic acid elabhorethri kanye nokungahambisani komtholampilo.

Izimiso zokutholwa kwe-SARS-CoV-2 nucleic acid

I-SARS-CoV-2 igciwane le-RNA elilandelana ngokulandelana kofuzo okungaba ngu-29 kb, elinezakhi zofuzo eziyi-10, ezingafaka ngempumelelo amaprotheni ayi-10.Amagciwane akhiwa i-RNA kanye namaprotheni, futhi ungqimba olungaphandle luyi-coating yangaphandle eyakhiwe ama-lipids nama-glycoprotein.Ngaphakathi, i-protein capsid igoqa i-RNA kuyo, ngaleyo ndlela ivikele i-RNA (P1) eyonakala kalula.

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I-P1 Isakhiwo se-SARS-COV-2

Amagciwane ahlasela amaseli ngokusebenzisa ama-receptors angaphezulu kweseli ukuze abangele ukutheleleka, futhi asebenzise amaseli abamba ukuze aziphindaphinde.

Umgomo wokutholwa kwe-viral nucleic acid uwukuveza i-RNA yegciwane ngeseli lysate, bese usebenzisa i-real-time fluorescent reverse transcription-polymerase chain reaction (RT-PCR) ukuze kutholwe.

Isihluthulelo somgomo wokutholwa ukusebenzisa ama-primers nama-probes ukuze kuzuzwe “ukufanisa okuhlosiwe” kokulandelana kwe-nucleic acid, okungukuthi, ukuthola ukulandelana kwe-nucleic acid ye-SARS-CoV-2 ehlukile kwamanye amagciwane ezisekelweni ezingaba ngu-30,000 (ukufana kwe-nucleic acid namanye amagciwane) indawo “ephansi”), ama-primers kanye nama-probes.

Ama-primers nama-probe afaniswa kakhulu nesifunda esithile se-SARS-CoV-2 nucleic acid, okungukuthi, ukucaciswa kunamandla kakhulu.Uma umphumela wokukhulisa i-fluorescent we-RT-PCR wesikhathi sangempela wesampula okufanele ihlolwe usulungile, kufakazela ukuthi i-SARS-CoV-2 ikhona kusampula.Bheka i-P2.

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Izinyathelo ze-P2 zokunqunywa kwe-SARS-CoV-2 nucleic acid (isikhathi sangempela se-fluorescent RT-PCR)

Izimo nezimfuneko zelabhorethri yokutholwa kwe-SARS-CoV-2 nucleic acid

Amalabhorethri okuhlola i-Nucleic acid yiwona alungele kakhulu izindawo zengcindezi engemihle, futhi kufanele anake ukuqapha ingcindezi, agcine umoya ugeleza, futhi asuse ama-aerosol.Abasebenzi abahlola i-Nucleic acid kumele babe neziqu ezihambisanayo, bathole ukuqeqeshwa okufanele kwe-polymerase chain reaction futhi baphumelele ukuhlolwa.Ilabhorethri kufanele ilawulwe ngokuqinile, ibekwe endaweni, futhi izisebenzi ezingabalulekile zivinjelwe ngokuphelele ukungena.Indawo ehlanzekile kufanele ifakwe umoya futhi ibulawe amagciwane endaweni.Izinto ezifanele zibekwe ezindaweni, ezihlanzekile nezingcolile ziyahlukaniswa, zishintshwe ngesikhathi, futhi zisuswe ukungcola endaweni.Ukubulala amagciwane okujwayelekile: Isibulala-magciwane esine-chlorine yisisombululo esikhulu sezindawo ezinkulu, kanti u-75% wotshwala ungasetshenziswa ezindaweni ezincane.Indlela enhle yokubhekana nama-aerosol ukuvula amafasitela ukuze kungene umoya, futhi ukukhishwa komoya-mpilo nakho kungenziwa ngemisebe ye-ultraviolet, ukuhlunga, nokubulala amagciwane emoyeni.

Izixhumanisi ezibalulekile namapharamitha we-SARS-CoV-2 nucleic acid determination (real-time fluorescent RT-PCR)

Nakuba amalabhorethri ngokuvamile enaka kakhulu “ukutholwa” kwe-nucleic acid, empeleni, “ukukhishwa” kwe-nucleic acid nakho kungenye yezinyathelo ezibalulekile zokutholwa ngempumelelo, okuhlobene eduze nokuqoqwa nokugcinwa kwamasampula egciwane.

Njengamanje, amasampula okuphefumula asetshenziswa kakhulu, njengama-nasopharyngeal swabs, asebenzisa indlela yesibili, okuyisisombululo sokungenzi lutho (sokulondoloza) esilungiselelwe ngokusekelwe ku-nucleic acid extraction kanye nesisombululo se-lysis.Ngakolunye uhlangothi, lesi sixazululo sokulondoloza igciwane singasusa amaprotheni egciwane, silahlekelwe umsebenzi walo futhi singabe sisabalele, futhi sithuthukise ukuphepha kwesiteji sokuthutha nokutholwa;ngakolunye uhlangothi, ingaqhekeza igciwane ngokuqondile ukuze ikhulule i-nucleic acid, iqede i-nucleic acid ebolayo i-enzyme, futhi ivimbele igciwane.I-RNA yehlisiwe.

Isixazululo sesampula segciwane esilungiselelwe ngesisekelo sesixazululo se-nucleic acid extraction lysis.Izingxenye eziyinhloko usawoti olinganiselayo, i-ethylenediaminetetraacetic acid chelating agent, usawoti we-guanidine (i-guanidine isothiocyanate, i-guanidine hydrochloride, njll.), i-anionic surfactant (dodecane) i-sodium sulfate), i-cationic surfactant (tetradecyl methyloxalate, i-hydroxyline, i-hydrochloride, i-hydrochloride, i-hydrochloride), proteinase K nezinye izingxenye eziningana noma ngaphezulu.Njengamanje, kunezinhlobo eziningi zekhithi yokukhipha i-nucleic acid, kanye ne-nucleic acid extraction kanye nama-reagents okuhlanza asetshenziswa.Ngisho noma kusetshenziswa i-nucleic acid efanayo kanye ne-reagent yokuhlanza, izinqubo zokukhipha ikhithi ngayinye zihlukile.

Okwamanje, imikhiqizo yekhithi yokuthola i-nucleic acid egunyazwe i-National Medical Products Administration ikhethwa ngokusekelwe ohlotsheni lofuzo lwe-ORF1ab, E no-N kugenome ye-SARS-CoV-2.Izimiso zokuthola imikhiqizo ehlukene ziyafana, kodwa ama-primers kanye nemiklamo ye-probe yehlukile.Kukhona amasegimenti okuqondiwe okukodwa (ORF1ab), amasegimenti athagethwe kabili (ORF1ab, N noma E), kanye namasegimenti amathathu okuqondiwe (ORF1ab, N kanye no-E).Umehluko phakathi kokutholwa nokuhumusha, ukukhishwa kwe-nucleic acid kanye nesistimu yokusabela ye-fluorescent ye-RT-PCR yesikhathi sangempela kufanele ibhekisele emiyalweni yekhithi efanelekile, futhi kunconywa ukuthi abasebenzisi balandele ngokuqinile indlela yokuhumusha ecaciswe emiyalweni yekhithi ukuze kuchazwe.Izifunda ezivamile, ama-primer kanye nokulandelana kwe-probe okukhuliswe yi-real-time fluorescent RT-PCR kuboniswa ku-P3.

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I-P3 Indawo ye-amplicon ye-SARS-CoV-2 eqondiswe ku-genome kanye nokulandelana kwama-primers nama-probes

Ukuhunyushwa kwemiphumela yokunqunywa kwe-SARS-CoV-2 nucleic acid (Ri-al-Ti-fluorescent RT-PCR)

“Uhlelo Lokuvimbela Nokulawula Inyumoniya lwe-SARS-CoV-2 Infection (Ushicilelo Lwesibili)” okokuqala ngqa lwacacisa indlela yokwahlulela imiphumela yofuzo olulodwa lokukhulisa:

1. Ayikho i-Ct noma i-Ct≥40 enegethivu;

2. I-Ct<37 ilungile;

3. Inani le-Ct lika-37-40 liyindawo yesilinganiso esimpunga.Kunconywa ukuthi uphinde ukuhlola.Uma umphumela wokwenza kabusha i-Ct<40 kanye nejika lokukhulisa lineziqongo ezisobala, isampula ibhekwa njengephozithivu, ngaphandle kwalokho inegethivu.

Uhlelo lwesithathu lomhlahlandlela kanye noshicilelo lwesine lomhlahlandlela lwaqhubeka nale mibandela engenhla.Kodwa-ke, ngenxa yezinhloso ezihlukene ezisetshenziswa kumakhithi ezentengiselwano, uhlelo lwesi-3 olushiwo ngenhla lomhlahlandlela aluzange lunikeze umbandela wokunquma inhlanganisela yokuhlosiwe, okugcizelela ukuthi Imiyalo enikezwe umenzi izophumelela.Kusukela kushicilelo lwesihlanu lweziqondiso, kucaciswe okubili okuhlosiwe, ikakhulukazi indlela yokwahlulela yenhloso eyodwa okunzima ukuyehlulela.Okusho ukuthi, uma ilabhorethri ifuna ukuqinisekisa ukuthi icala livuma ukutholwa kwe-nucleic acid yakwa-SARS-CoV-2, okulandelayo kudinga ukuhlangabezana nezimo ezi-1 kwezi-2:

(1) Okuhlosiwe okubili kwe-SARS-CoV-2 (ORF1ab, N) kusampula efanayo kuhlolwe ukuthi banesikhathi sangempela nge-fluorescent RT-PCR.Uma okuhlosiwe okukodwa kuvuma, ukuphinda kuthathwe isampula nokuhlolwa kabusha kuyadingeka.Uma imiphumela yokuhlolwa ithi.

(2) Amasampuli amabili e-RT-PCR ye-fluorescent yesikhathi sangempela abonise ithagethi eyodwa evumayo ngesikhathi esifanayo noma amasampula amabili ohlobo olufanayo abonisa umphumela wokuhlola onethagethi eyodwa, ongabhekwa njengephozithivu.Kodwa-ke, imihlahlandlela iphinde igcizelele ukuthi imiphumela emibi yokuhlolwa kwe-nucleic acid ayikwazi ukukhipha ukutheleleka kwe-SARS-CoV-2.Izinto ezingase zibangele ama-negative amanga adinga ukukhishwa, okuhlanganisa ikhwalithi yesampula empofu (amasampula okuphefumula avela ku-oropharynx nezinye izingxenye), ukuqoqwa kwesampula kusenesikhathi kakhulu noma sekwephuze kakhulu, Amasampula awazange agcinwe, athuthwe, futhi acutshungulwe ngendlela efanele, futhi ubuchwepheshe ngokwabo bube nezinkinga (ukuhlukahluka kwegciwane, ukuvinjelwa kwe-PCR), njll.

Izimbangela zokungalungi okungamanga ekutholweni kwe-SARS-CoV-2

Umqondo "we-negative wamanga" ekuhlolweni kwe-nucleic acid othintekayo njengamanje, uvame ukusho "okubi okungamanga" lapho imiphumela yokuhlolwa kwe-nucleic acid ingahambelani nokubonakaliswa komtholampilo, okungukuthi, izimpawu zomtholampilo nemiphumela yokucabanga kusolwa kakhulu nge-COVID-19, kodwa ukuhlolwa kwe-nucleic acid kuhlala "kubi" izikhathi eziningi.I-Clinical Laboratory Center yeKhomishana Yezempilo Kazwelonke ichaze ukuhlolwa kwe-SARS-CoV-2 “okungelona iqiniso”.

(1) Kunesilinganiso esithile segciwane emangqamuzaneni omuntu onaleli gciwane.Idatha ekhona ibonisa ukuthi ngemva kokuba umzimba utheleleke ngegciwane, igciwane lingena emphinjeni ngekhala nomlomo, bese lifika kuqhoqhoqho kanye ne-bronchi, bese lifika ku-alveoli.Umuntu onegciwane uzobhekana nesikhathi sokufukamela, izimpawu ezithambile, bese kuba nenqubo yezimpawu ezinzima, nezigaba ezihlukene zesifo.Futhi inani legciwane elikhona ezingxenyeni ezihlukene zomzimba lihlukile.

Ngokuphathelene nomthamo wegciwane wezinhlobo zamaseli, amaseli e-alveolar epithelial (umzila wokuphefumula ophansi)> amangqamuzana e-epithelial omoya (ipheshana lokuphefumula eliphezulu)> ama-fibroblasts, amaseli e-endothelial, nama-macrophages, njll.;kusukela ohlotsheni lwesampula, uketshezi lwe-alveolar lavage (Oluhle kakhulu)>isikhwehlela sokukhwehlela esijulile>nasopharyngeal swab>oropharyngeal swab>igazi.Ngaphezu kwalokho, igciwane lingatholakala nasendle.Nokho, uma kucatshangelwa ukuba lula kokuhlinza nokwamukela iziguli, isampula yesampula yomtholampilo esetshenziswa ngokuvamile i-oropharyngeal swab>nasopharyngeal swab>bronchial lavage fluid (ukuhlinzwa okuyinkimbinkimbi) nesikhwehlela esijulile (imvamisa ukukhwehlela okomile, okunzima ukukuthola) .

Ngakho-ke, inani legciwane kumaseli we-oropharynx noma i-nasopharynx yezinye iziguli lincane noma liphansi kakhulu.Uma kuphela amasampula e-oropharynx noma i-nasopharynx athathwa ukuze ahlolwe, i-viral nucleic acid ngeke itholakale.

(2) Awekho amaseli aqukethe igciwane aqoqwe ngesikhathi sokuqoqwa kwesampula, noma i-viral nucleic acid ayizange igcinwe ngokuphumelelayo.

[① Indawo yokuqoqa engalungile, isibonelo, uma uqoqa ama-oropharyngeal swabs, ukujula kweqoqo akwanele, ama-nasopharyngeal swabs aqoqwe ajule ekhaleni, njll. Iningi lamaseli aqoqiwe angaba amaseli angenalo amagciwane;

②Ama-swabs amasampula asetshenziswa ngokungalungile.Isibonelo, imicu yokwenziwa efana ne-PE fibre, i-polyester fiber kanye ne-polypropylene fiber inconywa ngezinto zekhanda le-swab.Imicu yemvelo efana nekotini isetshenziswa ekusebenzeni kwangempela (i-adsorption eqinile yamaprotheni futhi akulula ukuyigeza) Nemicu yenayiloni (ukumuncwa kwamanzi okungekuhle, okuholela ekulinganisweni kwevolumu enganele);

③Ukusetshenziswa okungalungile kwamashubhu okugcina amagciwane, okufana nokusetshenziswa kabi kwe-polypropylene noma amashubhu epulasitiki e-polyethylene amunca kalula ama-nucleic acid (DNA/RNA), okuholela ekwehleni kokugxiliswa kwe-nucleic acid esixazululweni sesitoreji.Empeleni, kutuswa ukusebenzisa ipulasitiki ye-polyethylene-propylene polymer nezinye iziqukathi zepulasitiki ze-polypropylene eziphathwe ngokukhethekile ukuze kugcinwe ama-nucleic acid anegciwane.]

[① Indawo yokuqoqa engalungile, isibonelo, uma uqoqa ama-oropharyngeal swabs, ukujula kweqoqo akwanele, ama-nasopharyngeal swabs aqoqwe ajule ekhaleni, njll. Iningi lamaseli aqoqiwe angaba amaseli angenalo amagciwane;

②Ama-swabs amasampula asetshenziswa ngokungalungile.Isibonelo, imicu yokwenziwa efana ne-PE fibre, i-polyester fiber kanye ne-polypropylene fiber inconywa ngezinto zekhanda le-swab.Imicu yemvelo efana nekotini isetshenziswa ekusebenzeni kwangempela (i-adsorption eqinile yamaprotheni futhi akulula ukuyigeza) Nemicu yenayiloni (ukumuncwa kwamanzi okungekuhle, okuholela ekulinganisweni kwevolumu enganele);

③Ukusetshenziswa okungalungile kwamashubhu okugcina amagciwane, okufana nokusetshenziswa kabi kwe-polypropylene noma amashubhu epulasitiki e-polyethylene amunca kalula ama-nucleic acid (DNA/RNA), okuholela ekwehleni kokugxiliswa kwe-nucleic acid esixazululweni sesitoreji.Empeleni, kutuswa ukusebenzisa ipulasitiki ye-polyethylene-propylene polymer nezinye iziqukathi zepulasitiki ze-polypropylene eziphathwe ngokukhethekile ukuze kugcinwe ama-nucleic acid anegciwane.]

(4) Ukusebenza kwelabhorethri yasemtholampilo akumisiwe.Izimo zesampula zokuhamba nezokugcina, ukusebenza okujwayelekile kwamalabhorethri emitholampilo, ukuchazwa kwemiphumela kanye nokulawulwa kwekhwalithi yizici ezibalulekile zokuqinisekisa ukunemba nokuthembeka kwemiphumela yokuhlolwa.Ngokwemiphumela yokuhlolwa kwekhwalithi yangaphandle okwenziwa yi-Clinical Laboratory Center ye-National Health Commission ngoMashi 16-24, 2020, kumalabhorethri angama-844 athole imiphumela esebenzayo, angama-701 (83.1%) aqeqeshiwe, kanti ayi-143 (16.9%) ayengafaneleki.Ifanelekile, izimo zokuhlola ilabhorethri zizonke zinhle, kodwa amalabhorethri ahlukene asenomehluko emandleni okusebenza kwabasebenzi, ikhono lokuchaza isampula eliqondile lesampula eliphokophelwe kanye nokulawulwa kwekhwalithi.

Ungawehlisa kanjani i-negetive engemihle yokutholwa kwe-SARS-CoV-2 nucleic acid?

Ukunciphisa ama-negetive angamanga ekutholweni kwe-nucleic acid kufanele kuthuthukiswe kusukela ezicini ezine zokukhiqiza okungekuhle okungamanga.

(1)Kunenani elithile legciwane kumaseli omuntu onaleli gciwane.Ukugcwala kwegciwane ezingxenyeni ezihlukene zomzimba wabantu okusolakala ukuthi banegciwane kuzohluka ngezikhathi ezahlukene.Uma kungekho pharynx, kungase kube ku-bronchial lavage fluid noma indle.Uma izinhlobo eziningi zamasampuli zingaqoqwa ngesikhathi esisodwa noma ezigabeni ezihlukene zokuqhubekela phambili kwesifo ukuze zihlolwe, Izosiza ekugwemeni amagethivu angamanga.

(2) Amaseli aqukethe igciwane kufanele aqoqwe ngesikhathi sokuqoqwa kwesampula.Le nkinga ingaxazululwa ngezinga elikhulu ngokuqinisa ukuqeqeshwa kwabaqoqi besampula.

(3) Ama-reagents e-IVD athembekile.Ngokwenza ucwaningo ngokuhlolwa kokusebenza kokutholwa kwama-reagents ezingeni likazwelonke, nokuxoxa ngezinkinga ezikhona, ukusebenza kahle kokutholwa kwama-reagents kungathuthukiswa futhi nokuzwela kokuhlaziya kungathuthukiswa.

(4) Ukusebenza okujwayelekile kwama-laboratories emitholampilo.Ngokuqinisa ukuqeqeshwa kwezisebenzi zaselabhorethri, ngokuqhubekayo ukuthuthukisa uhlelo lokuphathwa kwekhwalithi yaselabhorethri, ukuqinisekisa ukuhlukana okunengqondo, nokuthuthukisa ikhono lezisebenzi lokubona, kungenzeka ukunciphisa amanga amanga ngenxa yokusebenza okungafanele kwelabhorethri.

Izizathu zokuhlolwa kabusha kokuhlolwa kwe-SARS-CoV-2 nucleic acid ezigulini ezilulame nezilahliwe

I-“COVID-19 Diagnosis and Treatment Plan (Trial Seventh Edition)” ikubeka ngokusobala ukuthi enye yezindlela zokuthi iziguli ze-COVID-19 zelashwe futhi zikhishwe esibhedlela ukuthi amasampula epheshana lokuphefumula amabili alandelanayo anokuhlolwa okungeyikho kwe-nucleic acid (okungenani amahora angama-24 ngokuhlukana), kodwa mancane kakhulu I-SARS-CoV-2 iphinde yahlolwa nge-nucleic acid ngenxa yezizathu ezihlukahlukene.

(1)I-SARS-CoV-2 igciwane elisha.Kuyadingeka ukuqhubeka nokuqonda indlela yayo ye-pathogenic, isithombe esigcwele sesifo esibangelwe kanye nezici zenkambo yesifo.Ngakho-ke, ngakolunye uhlangothi, kuyadingeka ukuqinisa ukuphathwa kweziguli ezikhishwe futhi kubhekwe izinsuku eziyi-14 zezokwelapha.Ukulandelela, ukuqapha impilo kanye neziqondiso zezempilo ukuze ujulise ukuqonda kwayo yonke inqubo yokwenzeka, ukuthuthukiswa kanye nomphumela wesifo.

(2)Isiguli singase siphinde singenwe yileli gciwane.U-Academician Zhong Nanshan uthe: Ngenxa yokuthi iziguli ezelashiwe zinamasosha omzimba, i-SARS-CoV-2 ingaqedwa ngamasosha omzimba lapho ehlasela futhi.Ziningi izizathu, okungaba yimbangela yesiguli eseluleme, noma kungase kuhlobane nokuguqulwa kwegciwane, noma imbangela yokuhlolwa kwaselabhorethri.Uma kuyigciwane ngokwalo, ukuguqulwa kwe-SARS-CoV-2 kungabangela ukuthi i-antibody ekhiqizwa isiguli esilulamile ingasebenzi ngokumelene negciwane eliguquliwe.Uma isiguli sithelelekile ngegciwane eliguquliwe futhi, ukuhlolwa kwe-nucleic acid kungase kuphinde kube nethemba.

(3)Mayelana nezindlela zokuhlola elabhorethri, indlela ngayinye yokuhlola inemikhawulo yayo.Ukutholwa kwe-SARS-CoV-2 nucleic acid kungenxa yokukhethwa kokulandelana kofuzo, ukwakheka kwama-reagents, ukuzwela kwendlela nezinye izizathu, okuholela ekutheni amakhithi akhona abe nemikhawulo yawo yokuthola ephansi.Ngemva kokwelashwa kwesiguli, igciwane emzimbeni liyehla.Uma inani legciwane egazini kusampula ezohlolwa lingaphansi komkhawulo ophansi wokutholwa, umphumela "omubi" uzovela.Nokho, lo mphumela awusho ukuthi igciwane emzimbeni selinyamalele ngokuphelele.Igciwane lingase libe ngemva kokumiswa kokwelashwa.Resurgence”, qhubeka nokukopisha.Ngakho-ke, kunconywa ukuthi ubuyekeze kanye ngesonto phakathi kwamaviki angu-2 kuya kwangu-4 ngemva kokukhishwa.

(4) I-Nucleic acid iyizakhi zofuzo zegciwane.Igciwane libulawa ngemva kokuba isiguli selashwe nge-antiviral, kodwa izingcezu ze-RNA ezisele zegciwane zisagcinwe emzimbeni womuntu, futhi azikakhishwa ngokuphelele emzimbeni.Ngezinye izikhathi, ngaphansi kwezimo ezithile, ingagcinwa kakhudlwana.Isikhathi eside, futhi ngalesi sikhathi ukuhlolwa kwe-nucleic acid kuyoba "isikhashana" esihle.Ngokunwetshwa kwesikhathi sokululama kwesiguli, ngemuva kokuthi izingcezu ezisele ze-RNA emzimbeni seziphelile kancane kancane, umphumela wokuhlolwa kwe-nucleic acid ungaphenduka ungemuhle.

(5) Umphumela wokuhlolwa kwe-nucleic acid we-SARS-CoV-2 ufakazela kuphela ubukhona noma ukungabikho kwe-RNA yegciwane, futhi awukwazi ukufakazela umsebenzi wegciwane nokuthi igciwane liyasuleleka yini.Kuyadingeka ukufakazela ukuthi isiguli esinokuhlolwa kwe-nucleic acid futhi sizoba umthombo wokutheleleka futhi.Kuyadingeka ukwenza isiko legciwane kumasampula omtholampilo futhi kutshalwe igciwane “bukhoma” ukufakazela ukuthi liyathelelana.

Isifinyezo

Kafushane, i-SARS-CoV-2 nucleic acid ihlola ama-negatives angamanga, imiphumela yokuhlolwa kabusha, nezinye izimo ezingahambisani nokubonakaliswa komtholampilo azikwazi ukugwenywa ngokuphelele.Ekuhlolweni nasekuhlolweni kwangempela, kutuswa ukuba kuhlanganiswe izimpawu zomtholampilo, ukuhlolwa kwe-imaging (CT) kanye nokuhlolwa kweLabhorethri (ukuhlolwa kwe-nucleic acid + ukuhlolwa kwe-antibody okuqondene negciwane) imiphumela yokuxilongwa okuphelele ukuze kuvinjelwe ukuxilongwa okugejiwe kanye nokuxilongwa okungalungile.Uma imiphumela yokuhlolwa itholakala ngokusobala ukuthi ayihambisani nokubonakaliswa komtholampilo, kunconywa ukuba kwenziwe ukuhlaziya okuphelele kwaso sonke isixhumanisi sokuhlola (iqoqo lesampula, izixhumanisi zokujikeleza nokucubungula) ukuze kukhishwe ukutheleleka kwegciwane le-SARS-CoV-2 kusenesikhathi, ukutheleleka okuphindaphindiwe noma kuhlanganiswe nezinye izifo zegciwane lokuphefumula, njll.Uma izimo zivuma, kutuswa ukuba kuqoqwe amasampula azwela kakhulu njengesikhwehlela noma uketshezi lokuhlanza i-alveolar ukuze luphinde luhlolwe.

Imikhiqizo Ehlobene:

I-SARS-CoV-2 Nucleic Acid Detection Kit (I-Multiplex PCR Fluorescent Probe Method)


Isikhathi sokuthumela: Sep-03-2021