Abahlinzeki be-reagent ye-Molecular Biology kanye Nemboni |Abakhiqizi be-reagent ye-China Molecular Biology

I-Reagent ye-Molecular Biology

Uchungechunge lokuhlukaniswa kwe-RNA

Uchungechunge lokuhlukaniswa kwe-DNA

Uchungechunge oluqondile lwe-RT-qPCR

Uchungechunge lwe-PCR oluqondile lwegazi

Tshala uchungechunge lwe-PCR oluqondile

Uchungechunge lwe-PCR oluqondile lwezilwane

PCR/RT-PCR uchungechunge

Izinto zokusetshenziswa ze-IVD

Ikhithi encane ye-DNA yegazi

Hlanza ngokushesha i-genomic DNA yekhwalithi ephezulu egazini elinqanyuliwe (<1ml).

◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.

◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.

◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.

◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.

uphenyo imininingwane
I-General Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits

Hlanza ngokushesha i-plasmid DNA yekhwalithi ephezulu kubhaktheriya eguquliwe ukuze uthole ukuhlolwa okujwayelekile kwebhayoloji yamangqamuzana njengokuguqulwa nokugaya kwe-enzyme.

◮Susa i-RNA ngaphandle kokwengeza i-RNase

◮Elula-i-centrifugation yenziwa ekamelweni lokushisa

◮Ngokushesha-ukuhlinzwa kungaqedwa ngemizuzu engama-20

◮Kuphephile-akukho reagent ephilayo esetshenzisiwe

◮Ukuhlanzeka okuphezulu—OD260/280≈1.7-1.9

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I-RT Easyᵀᴹ I Master Premix yokuhlanganiswa kwe-cDNA yomucu wokuqala

- Uhlelo olusebenzayo lokuhlehla okulotshiweyo, kuthatha imizuzu engama-25 kuphela ukuqedela ukuhlanganiswa kwe-cDNA yomucu wokuqala.

- High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.

- Isistimu yokubhala okuphambene inokuqina okuphezulu kokushisa, izinga lokushisa lokusabela lesistimu lingafika ku-50 ℃,futhi inokusebenza okuhle kokuhlehla kokuloba.

- Ngisho namasampula e-RNA angcolile (utshwala obufika ku-45%, usawoti we-guanidine ofika ku-750mM) angaba ngaphansi kokusabela okuhlanekezelwe okulotshiweyo.

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I-RT Easy II(ene-gDNase) I-Master Premix ye-cDNA yochungechunge lokuqala lwe-Real Time PCR ene-gDNase

-Ikhono elisebenzayo lokususa i-gDNA, engasusa i-gDNA kusifanekiso phakathi nemizuzu emi-2.

-Isistimu yokubhala ehlehlayo esebenza kahle, kuthatha imizuzu eyi-15 kuphela ukuqeda ukuhlanganiswa kwe-cDNA yomucu wokuqala.

-Izifanekiso eziyinkimbinkimbi: izifanekiso ezinokuqukethwe okuphezulu kwe-GC nesakhiwo sesibili esiyinkimbinkimbi nazo zingahlehliswa ngokusebenza kahle okuphezulu.

-I-High-sensitivity reverse transcription system, izifanekiso zezinga le-pg nazo zingathola i-cDNA yekhwalithi ephezulu.

-I-reverse transcription system inokuqina okuphezulu kwe-thermal, izinga lokushisa elilungile lokusabela lingu-42℃, futhi isenokusebenza okuhle kokulotshiweyo okuhlehla kokungu-50℃.

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Isikhathi Sangempela PCR Easyᵀᴹ-SYBR Green I kit

I-Simple—2X PCR Mix ukuze kwehliswe iphutha lokuhlola nesikhathi sokusebenza

I-Specific-optimized buffer kanye ne-hot-start Taq enzyme ingavimbela ukukhulisa okungaqondile kanye nokwakheka kwe-primer dimer

Ukuzwela okuphezulu-kungathola amakhophi aphansi esifanekiso

Ukuguquguquka okuhle—kuhambisana nezinsimbi eziningi zesikhathi sangempela ze-PCR

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Ikhithi Yokuhlukanisa Yezilwane Ingqikithi Ye-RNA Ingqikithi Yekhithi Ye-RNA Yokukhipha Nezihlanzo Zezicubu Zezilwane Neseli

Khipha ngokushesha nangempumelelo ukuhlanzeka okuphezulu nekhwalithi ephezulu ingqikithi ye-RNA ezicutshini zezilwane ezahlukahlukene.

Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.Lonke uhlelo alunayo i-RNase-Free

Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column

Susa i-DNA ngaphandle kokwengeza i-DNase

Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi

Ukusheshisa—ukusebenza kungaqedwa ngemizuzu engu-30

Iphephile—ayikho i-organic reagent esetshenzisiwe

Ukuhlanzeka okuphezulu—OD260/280≈1.8-2.1

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Isethi Yekhithi Yokuzihlukanisa Yeseli Ye-RNA Ingqikithi Yekhithi Yokuhlanzwa Yokuzihlukanisa ye-RNA kuseli

I-RNA ehlanzwe kakhulu futhi yekhwalithi ephezulu ingatholakala kumaseli ahlukahlukene akhuliswe ngemizuzu eyi-11.

I-RNase-Free

Isebenza ekamelweni lokushisa (15-25 ℃)

Ngekholomu Yokuhlanza I-DNA

Inani eliphakeme kakhulu lama-RNA

Ukushesha:Qeda ukukhipha emizuzwini eyi-11

Ukuphepha:Ayikho i-Organic chemical

uphenyo imininingwane
I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi Kits ye-DNA Egazini

Hlanza ngokushesha i-genomic DNA yekhwalithi ephezulu egazini elinqanyuliwe (1-5ml).

◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.

◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.

◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.

◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.

uphenyo imininingwane
I-Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction noma Ikhithi Yokuhlanzwa ku-Buccal Swabs

Hlanza ngokushesha i-DNA yezinga eliphezulu ye-genomic kusuka kumasampula e-buccal swab/FTA Card.

◮Akukho ukungcoliswa kwe-RNase:Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukuvimbela ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

◮Isivinini esisheshayo:I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi kwemizuzu engu-20-80.

◮Kulula:I-centrifugation yenziwa ekamelweni lokushisa, futhi asikho isidingo se-centrifugation yezinga lokushisa eliphansi elingu-4°C noma imvula ye-ethanol ye-DNA.

◮Ukuphepha:Akukho kukhishwa kwe-organic reagent edingekayo.

◮Ikhwalithi ephezulu:I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, okungahlangabezana nezidingo zokuhlola okuhlukahlukene.

◮Isistimu ye-Micro-elution:Ingakhuphula ukugxila kwe-genomic DNA, elungele ukutholwa komfula noma ukuhlolwa.

uphenyo imininingwane
I-Foregene DNA Identification System 27Y

I-ForegeneI-DNA Identification System 27Y isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ukuze ikhulise ama-loci angu-27 Y-STR ngesikhathi esisodwa.Kufaka phakathi: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS39, DY39, DY39, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Ikhithi inekhono elihle lokuvimbela inhibition, futhi ingakhulisa ngokuqondile iphepha lokuhlunga, ikhadi le-FTA, i-cotton swab, ikhadi lamathe, i-oral swab, futhi ingasetshenziswa ukukhipha nokuhlanza izifanekiso ze-DNA ezintweni zamacala.

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I-Foregene DNA Identification System 38Y-C (Ikesi)

I-ForegeneUhlelo lokuhlonza i-DNA 38Y-U-C usebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuze akhulise 38 Y chromosome STR loci , 3 Y-Indel locifuthiizindawo ezimbili zokulawula ikhwalithi yangaphakathi ngesikhathi esisodwaukuze kukhuliswe Amasampula ekesi akhishwe kuzifanekiso ze-DNA ezihlanzekile.

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I-Foregene DNA Identification System 30A (Engakhishiwe)

FOREGENE DNA identification system 30A isebenzisa ubuchwepheshe bokulebula obunemibala eyisithupha ye-fluorescent ukuzekhulisa 29i-autosomalSIndawo ye-TR, eyodwaI-Amel locus kanye ne-Yindel locus eyodwa ngesikhathi esisodwa, futhi ingakhulisa ngokuqondile iphepha lokuhlunga, i-FTAamakhadi, ama-swabs kakotini, amakhadi amathe,futhi mi-outh swab.

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