I-China Incazelo ephezulu I-China Nucleic Acid Purification Extraction Yc-701 Cov Nucleic Acid Manufacturer and Supplier |I-Foregene

Ukuchazwa okuphezulu kwe-China Nucleic Acid Purification Extraction Yc-701 Cov Nucleic Acid

Incazelo yekhithi:

Le khithi isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic kumasampula ezicubu zezilwane ukuze kutholwe ukusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.

Inqubo yokukhipha i-DNA ye-genomic ku-lysis buffer iqedwa phakathi kwemizuzu eyi-10-30 ku-65.°C. Azikho ezinye izinqubo ezifana nokususwa kwamaprotheni ne-RNA ezidingekayo, futhi i-trace DNA ekhululiwe ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.

2×I-PCR elula^TMI-Mix (UNG) isebenzisa i-dUTP esikhundleni se-dTTP ngesisekelo esingu-2×I-PCR elula^TMHlanganisa, bese wengeza i-UNG enzyme (Uracil-N-glycosylase) engehlisa isithunzi isifanekiso esiqukethe i-dUTP ngesikhathi esifanayo.Ngaphambi kokusabela kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukuthunaza umkhiqizo we-PCR oqukethe i-uracil.I-enzyme ye-UNG ngeke ibe namuphi umthelela kusifanekiso esingaqukethe i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa futhi ivimbele ukuthi kungenzeka ukungcoliswa kwemikhiqizo ye-PCR ngesikhathi sokuhlolwa kofuzo okukhulu.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Sinamathela kumgomo othi "ikhwalithi kuqala, isevisi kuqala, intuthuko eqhubekayo kanye nokusungula izinto ezintsha ukuze sihlangabezane namakhasimende" kubaphathi kanye "nokungabi nasici, izikhalazo ezingenakho" njengenhloso yekhwalithi.Ukuze isevisi yethu iphelele, sinikeza imikhiqizo ngekhwalithi enhle ngentengo efanelekile yencazelo Ephezulu ye-China Nucleic Acid Purification Extraction Yc-701 Cov Nucleic Acid, Ukugcizelelwa okukhethekile ngokupakishwa kwezinto ezithengiswayo ukugwema noma yimuphi umonakalo ngesikhathi sokuthutha,Intshisekelo enemininingwane mayelana nempendulo ewusizo namasu abathengi bethu abahlonishwayo.
Sinamathela kumgomo othi "ikhwalithi kuqala, isevisi kuqala, intuthuko eqhubekayo kanye nokusungula izinto ezintsha ukuze sihlangabezane namakhasimende" kubaphathi kanye "nokungabi nasici, izikhalazo ezingenakho" njengenhloso yekhwalithi.Ukuze siphelelise isevisi yethu, sinikeza imikhiqizo ngekhwalithi enhle ngentengo efanelekileUhlelo lwe-China Nucleic Acid Extraction System, I-Nucleic Acid Extraction Instrument, Siya ngokuya sinweba isabelo sethu semakethe yamazwe ngamazwe ngokusekelwe ezintweni zekhwalithi, isevisi enhle kakhulu, intengo enengqondo nokulethwa ngesikhathi.Sicela usithinte nganoma yisiphi isikhathi ukuze uthole ulwazi olwengeziwe.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	Isivimbeli AL
	I-Foregene Protease
	I-6× I-DNA Yokulayisha Ibhafa
Ingxenye II	2×PCR Kulula^TMImiksi(UNG)

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo

∎ Isidingo sesampula sincane, njengoba izicubu zezilwane ezingama-5mg zingahlolwa.

■ Akukho ukwelashwa okukhethekile okufana nokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

■ Uhlelo lwe-PCR olulwa nokungcola 2×PCR Lulula^TMI-Mix (UNG) iqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekisa ukucaciswa nokunemba kokukhulisa.

Imingcele yekhithi

Isicelo: izicubu ezihlukahlukene zezilwane.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

i-wk flw-animal tissue direct PCR

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer AL ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

I-Reagent Foregene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-Reagent 2 × PCR Easy^TMI-Mix(UNG), uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).Sinamathela kumgomo othi "ikhwalithi kuqala, isevisi kuqala, ukuthuthukiswa okuqhubekayo kanye nokusha ukuze kuhlangatshezwane namakhasimende" kubaphathi kanye "neziro zesici, izikhalazo ezingenalutho" njengenhloso yekhwalithi.Ukuze isevisi yethu iphelele, sinikeza imikhiqizo ngekhwalithi enhle ngentengo efanelekile yencazelo Ephezulu ye-China Nucleic Acid Purification Extraction Yc-701 Cov Nucleic Acid, Ukugcizelelwa okukhethekile ngokupakishwa kwezinto ezithengiswayo ukugwema noma yimuphi umonakalo ngesikhathi sokuthutha,Intshisekelo enemininingwane mayelana nempendulo ewusizo namasu abathengi bethu abahlonishwayo.
Ukuchazwa okuphezuluUhlelo lwe-China Nucleic Acid Extraction System, I-Nucleic Acid Extraction Instrument, Siya ngokuya sinweba isabelo sethu semakethe yamazwe ngamazwe ngokusekelwe ezintweni zekhwalithi, isevisi enhle kakhulu, intengo enengqondo nokulethwa ngesikhathi.Sicela usithinte nganoma yisiphi isikhathi ukuze uthole ulwazi olwengeziwe.

Okwedlule: I-China Virus Nucleic Extraction Kit Yebanga Eliphezulu, Ibhokisi Lokuhlola I-Nucleic Acid, I-Nucleic Acid

Olandelayo: Intengo ephusile ye-China Real Time Fluorescence Virus Nucleic Acid PCR Test

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