I-Blood SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Blood Direct PCR Master Mix ye-Genotyping of Blood
Incazelo yekhithi:
Sebenzisa ngokuqondile igazi eliphelele le-heparin anticoagulated njengesifanekiso sokukhulisa i-PCR, esinokuzwela okuphezulu nokusebenza kahle kokukhulisa amandla.Uhlelo lwe-UNG lokulwa nokungcoliswa kwemvelo lwengezwe ukuze kuvinjelwe imibono engamanga.
◮Isistimu inekhono eliqinile lokukhulisa futhi ingakwazi ukubona ngokuzwela i-genome nezingcezu ze-DNA eziqondiswe kwamanye amazwe egazini.
◮2 × SuperEasyTMI-Mix(UNG)-Heparin Ukubekezelela igazi okuphezulu: kufika ku-45% egazini lomuntu kanye no-20% egazini legundane.
◮Isampula livalwe ngokugcwele, ngakho-ke asikho isidingo sokukhathazeka mayelana nokungcoliswa kwesampula kanye nemiphumela engelona iqiniso ye-PCR.Uhlelo lwe-PCR olulwa nokungcola
◮2 × SuperEasyTMHlanganisa (UNG), ukuqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR, futhi uqinisekise ukucaciswa nokunemba kokukhulisa.
Imininingwane
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMI-Mix(UNG)-Heparin: Ngesisekelo se-2× SuperEasyTMI-Mix-Heparin, i-dUTP isetshenziswa esikhundleni se-dTTP, futhi i-enzyme ye-UNG ekwazi ukwehlisa isifanekiso se-dUTP iyengezwa ngesikhathi esifanayo, engavimbela ngempumelelo ukungcoliswa kwemikhiqizo yokukhulisa i-PCR.Iqukethe i-D-Taq DNA Polymerase elungiswe ngokukhethekile, i-dNTPs, i-MgCl2, i-react buffer, i-PCR reaction enhancer, i-optimizer ne-stabilizer, njll. Ekuphenduleni kwe-PCR, vele wengeze igazi eliphelele elinqanda ukucoagulate, ama-primer, ne-ddH.2O kuya ku-2× SuperEasyTM Mix(UNG)-Heparin ehambisanayo ezosetshenziswa ekuphenduleni kwe-PCR.
I-6× I-DNA Loading Buffer: I-Loading Buffer ayiqukethe i-SDS.Kunconywa ukusebenzisa i-6× DNA Loading Buffer efakwe nekhithi uma wenza i-agarose gel electrophoresis ukuze uthole imiphumela emihle ye-electrophoresis.Ungasebenzisi i-Loading Buffer equkethe i-SDS, ngaphandle kwalokho kuzoba nomsila omkhulu wokukhanya okukhanyayo emzileni ngesikhathi se-electrophoresis, okuzothinta imiphumela yokuhlola.
Izingxenye zekhithi
| 2 × SuperEasyTMImiksi(UNG)-Heparin |
| I-6× I-DNA Yokulayisha Ibhafa |
| Iziyalezo |
Izici&izinzuzo
-Isistimu inekhono eliqinile lokukhulisa futhi ingakwazi ukubona ngokuzwela i-genome nezingcezu ze-DNA eziqondiswe kwamanye amazwe egazini.
-2 × SuperEasyTMI-Mix(UNG)-Heparin Ukubekezelela igazi okuphezulu: kufika ku-45% egazini lomuntu kanye no-20% egazini legundane.
-Isampula ivalwe ngokugcwele, ngakho-ke asikho isidingo sokukhathazeka mayelana nokungcoliswa kwesampula kanye nemiphumela engelona iqiniso ye-PCR.Uhlelo lwe-PCR olulwa nokungcoliswa kwe-2 × SuperEasyTMHlanganisa (UNG), ukuqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR, futhi uqinisekise ukucaciswa nokunemba kokukhulisa.
Isicelo sekhithi
Kunikezelwe ekuhlonzeni okuqondile kwe-PCR ye-Heparin anticoagulated lonke igazi.(Kuhlanganisa: igazi lomuntu, igazi legundane, igazi lenkukhu, igazi lenyoni, igazi lenkomo, igazi lenja, njll.)
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
Yonke inqubo yokuthutha kwebhokisi leqhwa elinezinga lokushisa eliphansi, ukuqinisekisa ukuthi ikhithi isesimweni esingu-<4 ℃.
2 × SuperEasyTMI-Mix(UNG)-I-Heparin, uma isetshenziswa njalo, ingabuye igcinwe ku-4°C ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi nezinsuku eziyi-10).
I-6× DNA Loading Buffer, ingagcinwa ku-4°C noma -20°C isikhathi eside.
