I-Blood SuperDirectᵀᴹ PCR Kit-Heparin Blood Direct PCR Master Mix ye-Genotyping of Blood
Incazelo yekhithi:
Sebenzisa ngokuqondile igazi eliphelele le-heparin anticoagulated njengesifanekiso sokukhulisa i-PCR, esinokuzwela okuphezulu nokusebenza kahle kokukhulisa amandla.
◮Asikho isidingo sokuhlanzwa kwe-DNA okuthatha isikhathi esiningi nokubizayo, akukho ukwelashwa kusengaphambili, nokusetshenziswa okuqondile kwegazi eliphelele njengesifanekiso se-PCR.
◮Isistimu inekhono eliqinile lokukhulisa futhi ingakwazi ukubona ngokuzwela i-genome nezingcezu ze-DNA eziqondiswe kwamanye amazwe egazini.
◮2 × SuperEasyTMI-Mix-Heparin High blood tolerance: kufika ku-45% egazini lomuntu kanye no-20% egazini legundane.
◮Isampula livalwe ngokugcwele, ngakho-ke asikho isidingo sokukhathazeka mayelana nokungcoliswa kwesampula kanye nemiphumela engelona iqiniso ye-PCR.
Imininingwane
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMI-Mix-Heparin:Iqukethe i-D-Taq DNA Polymerase elungiswe ngokukhethekile, i-dNTPs, i-MgCl2, i-react buffer, i-PCR reaction enhancer, i-optimizer, i-stabilizer, njll. Ekuphenduleni kwe-PCR, vele wengeze igazi eliphelele elinqanda ukucoagulate, iziqalo, ne-ddH.2O ku-2× SuperEasy ehambisanayoTMI-Mix-Heparin izosetshenziswa ekuphenduleni kwe-PCR.
I-6× I-DNA Loading Buffer:I-Buffer Yokulayisha ayinayo i-SDS.Kunconywa ukusebenzisa i-6× DNA Loading Buffer efakwe nekhithi uma wenza i-agarose gel electrophoresis ukuze uthole imiphumela emihle ye-electrophoresis.Ungasebenzisi i-Loading Buffer equkethe i-SDS, ngaphandle kwalokho kuzoba nomsila omkhulu wokukhanya okukhanyayo emzileni ngesikhathi se-electrophoresis, okuzothinta imiphumela yokuhlola.
Izingxenye zekhithi
| I-2 × I-SuperEasyTM Mix-Heparin |
| I-6× I-DNA Yokulayisha Ibhafa |
| Iziyalezo |
Izici&izinzuzo
-Asikho isidingo sokuhlanzwa kwe-DNA okuthatha isikhathi esiningi nokubizayo, akukho ukwelashwa kusengaphambili, nokusetshenziswa okuqondile kwegazi eliphelele njengesifanekiso se-PCR.
-Isistimu inekhono eliqinile lokukhulisa futhi ikwazi ukubona ngokuzwela i-genome nezingcezu ze-DNA eziqondiswe kwamanye amazwe egazini.
-2 × SuperEasyTMI-Mix-Heparin High blood tolerance: kufika ku-45% egazini lomuntu kanye no-20% egazini legundane.
-Isampula ivalwe ngokugcwele, ngakho-ke asikho isidingo sokukhathazeka mayelana nokungcoliswa kwesampula kanye nemiphumela engelona iqiniso ye-PCR.
Isicelo sekhithi
Kunikezelwe ekuhlonzeni okuqondile kwe-PCR ye-Heparin anti-coagulated lonke igazi.(Kuhlanganisa: igazi lomuntu, igazi legundane, igazi lenkukhu, igazi lenyoni, igazi lenkomo, igazi lenja, njll.)
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
Le kit kufanele igcinwe ku -20 ℃.
2 × SuperEasyTMI-Mix-Heparin，Uma isetshenziswa njalo, ingagcinwa ku-4°C ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).
I-6× DNA Loading Buffer, Ingagcinwa ku-4°C noma -20°C isikhathi eside.
