I-Blood SuperDirectᵀᴹ PCR Kit-EDTA Blood Direct PCR Master Mix ye-Genotyping of Blood
Imininingwane
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMI-Mix-EDTA: Iqukethe i-D-Taq DNA Polymerase elungiswe ngokukhethekile, i-dNTPs, i-MgCl2, i-react buffer, i-PCR reaction enhancer, i-optimizer ne-stabilizer, njll. Ekuphenduleni kwe-PCR, vele ungeze igazi eliphelele elinqanda ukucoagulate, iziqalo, ne-ddH.2O ku-2× SuperEasy ehambisanayoTMI-Mix-EDTA izosetshenziswa ekuphenduleni kwe-PCR.
I-6× I-DNA Loading Buffer: I-Loading Buffer ayiqukethe i-SDS.Kunconywa ukusebenzisa i-6× DNA Loading Buffer efakwe nekhithi uma wenza i-agarose gel electrophoresis ukuze uthole imiphumela emihle ye-electrophoresis.Ungasebenzisi i-Loading Buffer equkethe i-SDS, ngaphandle kwalokho kuzoba nomsila omkhulu wokukhanya okukhanyayo emzileni ngesikhathi se-electrophoresis, okuzothinta imiphumela yokuhlola.
Izingxenye zekhithi
2 × SuperEasyTMI-Mix-EDTA |
I-6× I-DNA Yokulayisha Ibhafa |
Iziyalezo |
Izici&izinzuzo
-Asikho isidingo sokuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo, akukho ukwelashwa kusengaphambili, nokusetshenziswa okuqondile kwegazi eliphelele njengesifanekiso se-PCR.
-Isistimu inekhono eliqinile lokukhulisa futhi ikwazi ukubona ngokuzwela i-genome nezingcezu ze-DNA eziqondiswe kwamanye amazwe egazini.
-2 × SuperEasyTMI-Mix-EDTA Ukubekezelela igazi okuphezulu: kufika ku-45% egazini lomuntu kanye no-20% egazini legundane.
-Isampula ivalwe ngokugcwele, ngakho-ke asikho isidingo sokukhathazeka mayelana nokungcoliswa kwesampula kanye nemiphumela engelona iqiniso ye-PCR.
Isicelo sekhithi
Kunikezelwe ekuhlonzeni okuqondile kwe-PCR ye-EDTA egazini eligcwele igazi.(Kuhlanganisa: igazi lomuntu, igazi legundane, igazi lenkukhu, igazi lenyoni, igazi lenkomo, igazi lenja, njll.)
Ukuhamba komsebenzi
Isitoreji kanye Nempilo yeshelufu
Le kit kufanele igcinwe ku -20°C.
2 × SuperEasyTMI-Mix-EDTA, uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).
I-6× DNA Loading Buffer, ingagcinwa ku-4°C noma -20°C isikhathi eside.